HowTo's and Tutorials

EasyTau
Interfacing a time-resolved spectrometer (FluoTime 300) with a microscope (MicroTime 100)
Measuring the Quantum Yield with the Integrating Sphere Assembly for the FluoTime 300

Configuring SymPhoTime64
FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade
FLIM Measurement Using a Zeiss LSM710/LSM780/LSM880 with a FLIM and FCS Upgrade
FLIM Measurements Using an Olympus FV3000 with a PicoQuant FLIM Upgrade
FLIM-FRET Measurement Using an Olympus FV1200 with a FLIM and FCS Upgrade
How to Avoid the Pile-up Effect in FLIM Measurements
How to Check the Overlap of Different Color Confocal Volumes
How to exchange the main dichroic of the MicroTime 200
How to Measure the Instrument Response Function (IRF)
How to Perform Antibunching Measurements
How-to select the correct pinhole size
Image on the beam diagnostics camera during focusing
MT200 Daily Alignment
MT200 FCS Measurement
MT200 Fundamental Alignment
Nikon AX: NIS-Elements FLIM Examples
Nikon AX: Phasor-Based Structural Separation Using FLIM
NovaFLIM Tutorials
Performing a FCS measurement with an Olympus FV1200 Upgrade Kit
Recording a Fluorescence Lifetime Image (FLIM) Stack with a LSM Upgrade Kit on a Nikon A1

NovaFLIM Tutorials

The SymPhoTime tutorials rely on the sample workspace downloadable here (1.5GB).

Antibunching Analysis
Determination of the Focal Width
How to create a time-gated Image in SymPhoTime
How to Work with Instrument Response Functions (IRFs) Measured with a Microscope
Intensity Time Trace Analysis
Registering New Scripts in the SymPhoTime 64
Static Anisotropy Analysis for Images
SymPhoTime 64 Analysis Tips and Tricks
SymPhoTime Lifetime Fitting

Lifetime Fitting Using the FLIM Analysis
Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above)
Lifetime-Fitting Using the Rapid Reconvolution Model
Pattern Matching
Phasor Analysis
ROI Fitting Using the FLIM Analysis
ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)
Visualizing Dynamics Using the Multi Frame FLIM Analysis
Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above)

Calculate Ratiometric FRET Images
Calculate Ratiometric Single Pair FRET Distributions
Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRET
FLIM-FRET Calculation for Multi Exponential Donors
FLIM-FRET Calculation for Single Exponential Donors

Calculate and Fit FCS Traces with the FCS Script
Calculate FCCS Traces with the Grouped FCS Script
Calibrate the Confocal Volume for and with FCS
Separation of 2 Species with Different Lifetimes Using FLCS
Spectral Crosstalk Removal via FLCCS

Measurement results obtained by FluoTime series spectrometers controlled with EasyTau 2 software are directly stored in a workspace in .etc data containers. This is also the case when TimeHarp260, PicoHarp300, HydraHarp400 or MultiHarp150 is controlled via EasyTau 2. Such .etc files can be opened directly, using the button.

It is also possible to use the native software of the timing electronics for data acquisition, instead of control via EasyTau 2. Recent versions of TimeHarp260.exe, PicoHarp.exe, HydraHarp.exe and MultiHarp.exe, started from Windows Start menu, store the histograms in .phu data format. Older software versions of various timing electronics may use other data file formats. Possible file name extensions are .thd, .nhd, .phd and .hhd In order to analyze such files, they have to be imported into an already existing EasyTau2 workspace.