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FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade

This tutorial shows the recording of FLIM images using an LSM upgrade kit, in this case a Nikon A1. In FLIM-measurements, the fluorescence lifetime of a component can be determined which can be used e.g.

  • to distinguish different tissue or cell constituents
  • to monitor concentration changes using a reporter dye
  • To monitor interactions between two molecules via FRET. Recording of FLIM images especially for

FLIM-FRET is also shown in a separate video (see link below).

The general principles of the data acquisition are analogous in all LSM upgrade kits. The movie was recorded in 02/2014 and shows the integration with Nikon A1 system with a NIS version lower than 4.3; the recording procedure may be somewhat different in higher or very old versions.

Update: NIS Elements 4.3 integrates control for FLIM and FCS

howto/flim_measurement_using_a_nikon_a1_with_a_flim_and_fcs_upgrade.txt · Last modified: 2015/06/30 15:07 by buschmann