FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade
This tutorial shows the recording of FLIM images using an LSM upgrade kit, in this case a Nikon A1. In FLIM-measurements, the fluorescence lifetime of a component can be determined which can be used e.g.
- to distinguish different tissue or cell constituents
- to monitor concentration changes using a reporter dye
- To monitor interactions between two molecules via FRET. Recording of FLIM images especially for
FLIM-FRET is also shown in a separate video (see link below).
The general principles of the data acquisition are analogous in all LSM upgrade kits. The movie was recorded in 02/2014 and shows the integration with Nikon A1 system with a NIS version lower than 4.3; the recording procedure may be somewhat different in higher or very old versions.
Update: NIS Elements 4.3 integrates control for FLIM and FCS
The movie was recorded in 02/2015; the recording procedure may be somewhat different in older or newer models.