howto:start

Table of Contents

HowTo's and Tutorials
- Instrumentation
  - Fluorometer
  - Microscopy
- Analysis
  - General
  - FLIM
  - FRET
  - FCS / Correlation
- Samples

HowTo's and Tutorials

Instrumentation

Fluorometer

EasyTau

Interfacing a time-resolved spectrometer (FluoTime 300) with a microscope (MicroTime 100)

Measuring the Quantum Yield with the Integrating Sphere Assembly for the FluoTime 300

Microscopy

Configuring SymPhoTime64

FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade

FLIM Measurement Using a Zeiss LSM710/LSM780/LSM880 with a FLIM and FCS Upgrade

FLIM Measurements Using an Olympus FV3000 with a PicoQuant FLIM Upgrade

FLIM-FRET Measurement Using an Olympus FV1200 with a FLIM and FCS Upgrade

How to Avoid the Pile-up Effect in FLIM Measurements

How to Check the Overlap of Different Color Confocal Volumes

How to exchange the main dichroic of the MicroTime 200

How to Measure the Instrument Response Function (IRF)

How to Perform Antibunching Measurements

How-to select the correct pinhole size

Image on the beam diagnostics camera during focusing

MT200 Daily Alignment

MT200 FCS Measurement

MT200 Fundamental Alignment

Performing a FCS measurement with an Olympus FV1200 Upgrade Kit

Recording a Fluorescence Lifetime Image (FLIM) Stack with a LSM Upgrade Kit on a Nikon A1

Analysis

The SymPhoTime tutorials rely on the sample workspace downloadable here (1.5GB).

General

Antibunching Analysis

Determination of the Focal Width

How to create a time-gated Image in SymPhoTime

How to Work with Instrument Response Functions (IRFs) Measured with a Microscope

Intensity Time Trace Analysis

Registering New Scripts in the SymPhoTime 64

Static Anisotropy Analysis for Images

SymPhoTime 64 Analysis Tips and Tricks

SymPhoTime Lifetime Fitting

FLIM

Lifetime Fitting Using the FLIM Analysis

Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above)

Lifetime-Fitting Using the Rapid Reconvolution Model

Pattern Matching

Phasor Analysis

ROI Fitting Using the FLIM Analysis

ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)

Visualizing Dynamics Using the Multi Frame FLIM Analysis

Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above)

FRET

Calculate Ratiometric FRET Images

Calculate Ratiometric Single Pair FRET Distributions

Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRET

FLIM FRET Calculation for Multi Exponential Donors

FLIM-FRET Calculation for Single Exponential Donors

FCS / Correlation

Calculate and Fit FCS Traces with the FCS Script

Calculate FCCS Traces with the Grouped FCS Script

Calibrate the Confocal Volume for and with FCS

Separation of 2 Species with Different Lifetimes Using FLCS

Spectral Crosstalk Removal via FLCCS

Samples

Diamond NV Centers

howto/start.txt · Last modified: 2020/04/03 10:25 by buschmann