Note: The integrating Sphere is made from Spectralon™. While this material provides excellent spectral reflectivity and spectral flatness it is also very sensitive to contamination. Due to the porous nature of its surface, it collects impurities easily. Any impurity will change the reflectivity of the inner walls of the integrating sphere and thus will influence the measurements. Therefore, handle the integrating sphere carefully avoiding any exposure to dust or other impurities. Avoid spilling sample into the sphere or contamination of the sample holder.
Note: The sample holder has two indexed positions. When in the IN position, the excitation beam will directly hit the sample, when in the OUT position the sample will only be illuminated indirectly (by diffuse light originating from the sphere's surface).
Note: In this example with Rh6G as the sample we will use 510nm for excitation, therefore the spectral range will be from 500 nm to 700 nm.
Note: The blank should have the same geometry as the sample. In our example this is a identical cuvette filled with the solvent (ethanol).
Note: The position should be the same as for the sample measurement.
Note: The values shown in the wizard may not be precise. The precise values are calculated when you reopen the saved QY measurement using the plot tool – see below.
Note: The name of the data file will contain the approximate QY value.