{{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the ... e FLIM analysis. Thereby, a new Window opens:
{{ howto:kidneyfullscreen3.png |}}
**Note:**\\
The window... I" checkbox and click "ROI from threshold".\\
{{ howto:activateroi.png |}}
**Response:**\\
A large win... a threshold for an image analysis visually.
{{ howto:selectthreshold_kidney3.png |}}
**Note:**\\
Lef
ications =====
==== Fluorescence Lifetime ====
[[howto:lifetime_fitting_using_the_tcpsc_fitting_script| ... video_tutorials| FLIM by Philippe Bastiaens]]
[[howto:how_to_measure_the_instrument_response_function_i... asure the Instrument Response function (IRF)]]
[[howto:lifetime_fitting_using_the_flim_analysis|Lifetime fitting Using the FLIM analysis software]]
[[howto:roi_fitting_using_the_flim_script|ROI fitting usi
{{tag> howto FLIM imaging ROI analysis tutorial SymPhoTime}}
~~NOTOC~~
====== ROI Fitting Using the FLI... pt. Also check the script explaining pixel fitting ([[howto:Lifetime Fitting using the FLIM Analysis]]).
{{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analys... rt the FLIM script by clicking on "Start".\\
{{ howto:startflim.png?200 |}}
**Response:**\\
The FL... lls_FLIM.ptu''. Thereby, a new Window opens:
{{ howto:kidneyfullscreen3.png |}}
**Note:**\\
The windo... channel 4 - and press "Calculate FastFLIM".\\
{{ howto:detectionchannel.png?400 |}}
**Response:**\\
Th
antfcs.pdf|Quantitative FCS measurements.]]
* [[howto:Calculate and Fit FCS Traces with the FCS Script|... FCS curves with the SymPhoTIme64 software]]
* [[howto:calibrate_the_confocal_volume_for_fcs_using_the_f
{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}
====== How to Work with Instrument Response F... ator the timing resolution of the instrument.
{{ howto:irf.png?500 |}}
The IRF width is approx. a quad... re described here: https://www.tcspc.com/doku.php/howto:how_to_measure_the_instrument_response_function_i... ral strategies are possible which are described [[howto:how_to_measure_the_instrument_response_function_i