TAG: howto

Antibunching Analysis	2014/02/19 07:20
Calculate and Fit FCS Traces with the FCS Script	2014/02/19 07:19
Calculate FCCS Traces with the Grouped FCS Script	2014/02/19 11:54
Calculate Ratiometric FRET Images	2014/02/19 06:34
Calculate Ratiometric Single Pair FRET Distributions	2014/02/19 07:20
Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRET	2014/02/19 07:20
Calibrate the Confocal Volume for and with FCS	2014/02/19 07:20
Configuring SymPhoTime64	2014/06/26 14:10	Evangelos Sisamakis
Determination of the Focal Width	2014/02/19 07:20
Diamond NV Centers	2013/08/01 08:45	Christina de Rosario
FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade	2014/06/18 16:29	Steffen Ruettinger
FLIM Measurement Using a Zeiss LSM710/LSM780/LSM880 with a FLIM and FCS Upgrade	2016/07/20 12:01	Volker Buschmann
FLIM Measurements Using an Olympus FV3000 with a PicoQuant FLIM Upgrade	2020/05/18 12:51	Volker Buschmann
FLIM-FRET Calculation for Multi Exponential Donors	2014/01/31 12:21
FLIM-FRET Calculation for Single Exponential Donors	2014/01/30 12:17	Steffen Ruettinger
FLIM-FRET Measurement Using an Olympus FV1200 with a FLIM and FCS Upgrade	2014/06/18 16:28
How to Avoid the Pile-up Effect in FLIM Measurements	2015/02/06 10:17	Steffen Ruettinger
How to Check the Overlap of Different Color Confocal Volumes	2013/08/06 14:51
How to create a time-gated Image in SymPhoTime	2020/10/14 11:45	Volker Buschmann
How to exchange the main dichroic of the MicroTime 200	2015/11/04 13:01	Evangelos Sisamakis
How to Measure the Instrument Response Function (IRF)	2013/11/27 09:54
How to Perform Antibunching Measurements	2013/08/01 11:37	Christina de Rosario
How to Work with Instrument Response Functions (IRFs) Measured with a Microscope	2020/04/15 07:58	Volker Buschmann
How-to select the correct pinhole size	2015/09/11 15:16	Steffen Ruettinger
Image on the beam diagnostics camera during focusing	2015/11/05 16:38	Evangelos Sisamakis
Intensity Time Trace Analysis	2014/05/06 11:26
Interfacing a time-resolved spectrometer (FluoTime 300) with a microscope (MicroTime 100)	2020/05/18 14:19	Volker Buschmann
Lifetime Fitting Using the FLIM Analysis	2014/02/19 07:47
Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above)	2020/04/09 15:52	Samaneh Rezvani
Lifetime-Fitting Using the Rapid Reconvolution Model	2020/04/02 12:14	Samaneh Rezvani
Measuring the Quantum Yield with the Integrating Sphere Assembly for the FluoTime 300	2014/06/18 12:42	Steffen Ruettinger
MT200 Daily Alignment	2021/06/25 09:01	Samaneh Rezvani
MT200 FCS Measurement	2021/06/25 09:04	Samaneh Rezvani
MT200 Fundamental Alignment	2021/06/25 08:43	Samaneh Rezvani
Nikon AX: NIS-Elements FLIM Examples	2025/06/16 08:46	Chenyang Lan
Nikon AX: Phasor-Based Structural Separation Using FLIM	2024/09/13 08:39	Chenyang Lan
NovaFLIM Tutorials	2024/12/19 15:55	Steffen Ruettinger
Pattern Matching	2014/02/19 07:20
Performing a FCS measurement with an Olympus FV1200 Upgrade Kit	2014/07/09 13:18
Phasor Analysis	2014/05/05 13:12	Steffen Ruettinger
Recording a Fluorescence Lifetime Image (FLIM) Stack with a LSM Upgrade Kit on a Nikon A1	2014/06/19 08:29	Steffen Ruettinger
Registering New Scripts in the SymPhoTime 64	2014/02/19 07:20
ROI Fitting Using the FLIM Analysis	2014/02/19 07:20
ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)	2023/02/23 06:15	Chenyang Lan
Separation of 2 Species with Different Lifetimes Using FLCS	2014/02/19 07:20
Spectral Crosstalk Removal via FLCCS	2014/02/19 07:20
Static Anisotropy Analysis for Images	2014/02/19 07:20
SymPhoTime 64 Analysis Tips and Tricks	2015/01/21 15:53	Steffen Ruettinger
SymPhoTime Lifetime Fitting	2014/02/19 07:20
Visualizing Dynamics Using the Multi Frame FLIM Analysis	2014/02/19 07:39
Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above)	2020/04/15 13:31	Samaneh Rezvani