TAG: howto

Antibunching Analysis	2014/02/19 08:20
Burst duration and FCS diffusion time	2026/01/12 11:06	Denis Doerr
Calculate and Fit FCS Traces with the FCS Script	2014/02/19 08:19
Calculate FCCS Traces with the Grouped FCS Script	2014/02/19 12:54
Calculate Ratiometric FRET Images	2014/02/19 07:34
Calculate Ratiometric Single Pair FRET Distributions	2014/02/19 08:20
Calculate Ratiometric Single Pair FRET Distributions Using PIE-FRET	2014/02/19 08:20
Calibrate the Confocal Volume for and with FCS	2014/02/19 08:20
Configuring SymPhoTime64	2014/06/26 16:10	Evangelos Sisamakis
Determination of the Focal Width	2014/02/19 08:20
Diamond NV Centers	2013/08/01 10:45	Christina de Rosario
FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade	2014/06/18 18:29	Steffen Ruettinger
FLIM Measurement Using a Zeiss LSM710/LSM780/LSM880 with a FLIM and FCS Upgrade	2016/07/20 14:01	Volker Buschmann
FLIM Measurements Using an Olympus FV3000 with a PicoQuant FLIM Upgrade	2020/05/18 14:51	Volker Buschmann
FLIM-FRET Calculation for Multi Exponential Donors	2014/01/31 13:21
FLIM-FRET Calculation for Single Exponential Donors	2014/01/30 13:17	Steffen Ruettinger
FLIM-FRET Measurement Using an Olympus FV1200 with a FLIM and FCS Upgrade	2014/06/18 18:28
How to Avoid the Pile-up Effect in FLIM Measurements	2015/02/06 11:17	Steffen Ruettinger
How to Check the Overlap of Different Color Confocal Volumes	2013/08/06 16:51
How to create a time-gated Image in SymPhoTime	2020/10/14 13:45	Volker Buschmann
How to exchange the main dichroic of the MicroTime 200	2015/11/04 14:01	Evangelos Sisamakis
How to Measure the Instrument Response Function (IRF)	2013/11/27 10:54
How to Perform Antibunching Measurements	2013/08/01 13:37	Christina de Rosario
How to Work with Instrument Response Functions (IRFs) Measured with a Microscope	2020/04/15 09:58	Volker Buschmann
How-to select the correct pinhole size	2015/09/11 17:16	Steffen Ruettinger
Image on the beam diagnostics camera during focusing	2015/11/05 17:38	Evangelos Sisamakis
Intensity Time Trace Analysis	2014/05/06 13:26
Interfacing a time-resolved spectrometer (FluoTime 300) with a microscope (MicroTime 100)	2020/05/18 16:19	Volker Buschmann
Lifetime Fitting Using the FLIM Analysis	2014/02/19 08:47
Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above)	2020/04/09 17:52	Samaneh Rezvani
Lifetime-Fitting Using the Rapid Reconvolution Model	2020/04/02 14:14	Samaneh Rezvani
Measuring the Quantum Yield with the Integrating Sphere Assembly for the FluoTime 300	2014/06/18 14:42	Steffen Ruettinger
MT200 Daily Alignment	2021/06/25 11:01	Samaneh Rezvani
MT200 FCS Measurement	2021/06/25 11:04	Samaneh Rezvani
MT200 Fundamental Alignment	2021/06/25 10:43	Samaneh Rezvani
Nikon AX: NIS-Elements FLIM Examples	2025/06/16 10:46	Chenyang Lan
Nikon AX: Phasor-Based Structural Separation Using FLIM	2024/09/13 10:39	Chenyang Lan
Pattern Matching	2014/02/19 08:20
Performing a FCS measurement with an Olympus FV1200 Upgrade Kit	2014/07/09 15:18
Phasor Analysis	2014/05/05 15:12	Steffen Ruettinger
Recording a Fluorescence Lifetime Image (FLIM) Stack with a LSM Upgrade Kit on a Nikon A1	2014/06/19 10:29	Steffen Ruettinger
Registering New Scripts in the SymPhoTime 64	2014/02/19 08:20
ROI Fitting Using the FLIM Analysis	2014/02/19 08:20
ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)	2023/02/23 07:15	Chenyang Lan
Separation of 2 Species with Different Lifetimes Using FLCS	2014/02/19 08:20
Spectral Crosstalk Removal via FLCCS	2014/02/19 08:20
Static Anisotropy Analysis for Images	2014/02/19 08:20
SymPhoTime 64 Analysis Tips and Tricks	2015/01/21 16:53	Steffen Ruettinger
SymPhoTime Lifetime Fitting	2014/02/19 08:20
Visualizing Dynamics Using the Multi Frame FLIM Analysis	2014/02/19 08:39
Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above)	2020/04/15 15:31	Samaneh Rezvani