Applications
Fluorescence Lifetime
Fluorescence Lifetime Imaging Microscopy (FLIM)
How to measure the Instrument Response function (IRF)
Lifetime fitting Using the FLIM analysis software
ROI fitting using FLIM analysis software
FLIM-FRET Calculation for Single Exponential Donors
Förster Resonance Energy Transfer (FRET)
Calculate ratiometric FRET-Images
Calculate ratiometric single pair FRET distributions
Calculate ratiometric single pair FRET distributions using Pulsed Interleaved Excitation (PIE)
Fluorescence Correlation Spectroscopy (FCS)
A short introduction to FCS with suggestions for further reading
Performing an FCS Measurement with an Olympus FV1200 upgrade kit
Calibrate the confocal volume using the FCS calibration analysis
Remove spectral crosstalk in dual color FCCS via FLCCS
Separation of 2 species with different lifetimes using FLCS