Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength. (Fluorescence)
Fluorescence and Fluorescence Microscopy explained by Nico Stuurman at UCSF.
Mark Armitage explains major components of confocal microscopes and their use.
This video is part of a series of videos. This is the related playlist: https://www.youtube.com/playlist?list=PLO0yDSkagDljzI0bdM5m1njrdLdVa80_N
Nikons website MicroscopyU.com has a very nice course on confocal microscopy.
Confocal Microscopy explained by Kurt Thorn of the Nikon imaging center at UCSF
Kurt Thorn of UCSF talks about two-photon microscopy which uses intense pulsed lasers to image deep into biological samples. It can be used for imaging thick tissue specimens or even imaging inside of live animals.
Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography. The lifetime of the fluorophore signal, rather than its intensity, is used to create the image in FLIM. This has the advantage of minimizing the effect of photon scattering in thick layers of sample ( FLIM).