This is an old revision of the document!
Applications
Fluorescence Lifetime
Fluorescence Lifetime Imaging Microscopy (FLIM)
How to measure the Instrument Response function (IRF)
Lifetime fitting Using the FLIM analysis software
ROI fitting using FLIM analysis software
FLIM-FRET Calculation for Single Exponential Donors
Förster Resonance Energy Transfer (FRET)
Calculate ratiometric FRET-Images
Calculate ratiometric single pair FRET distributions
Calculate ratiometric single pair FRET distributions using Pulsed Interleaved Excitation (PIE)
Fluorescence Correlation Spectroscopy (FCS)
Performing an FCS Measurement with an Olympus FV1200 upgrade kit
Calibrate the confocal volume using the FCS calibration analysis
Remove spectral crosstalk in dual color FCCS via FLCCS