FLIM, fast flim

Fast FLIM

FLIM image, using the fast lifetime calculation. The color-coded fast lifetime of each pixel represents the average photon arrival time with respect to the preceding laser pulse.

FLIM in this wiki

Fast FLIM

FLIM

FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade

FLIM Measurement Using a Zeiss LSM710/LSM780/LSM880 with a FLIM and FCS Upgrade

FLIM Measurements Using an Olympus FV3000 with a PicoQuant FLIM Upgrade

FLIM-FRET Calculation for Multi Exponential Donors

FLIM-FRET Calculation for Single Exponential Donors

FLIM-FRET Measurement Using an Olympus FV1200 with a FLIM and FCS Upgrade

How to Avoid the Pile-up Effect in FLIM Measurements

Interfacing a time-resolved spectrometer (FluoTime 300) with a microscope (MicroTime 100)

Lifetime Fitting Using the FLIM Analysis

Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above)

Lifetime-Fitting Using the Rapid Reconvolution Model

Pattern Matching

Phasor Analysis

Recording a Fluorescence Lifetime Image (FLIM) Stack with a LSM Upgrade Kit on a Nikon A1

ROI Fitting Using the FLIM Analysis

ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)

Visualizing Dynamics Using the Multi Frame FLIM Analysis

Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above)