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avoid_pile_up_effect_in_flim_measurements @howto
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{{tag> howto FLIM acquisition pile-up TCSPC}} ~~TOC~~ ====== How to Avoid the Pile-up Effect in FLIM Measurements ====== For a definition of the pile-up effect see here: [[glossary:pile-up_effect|Pile-Up Effect]] To avoid [[glossary:pile-up_effect|pile-
pile-up_effect @glossary
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{{tag> TCSPC pile-up dead_time}} ~~TOC~~ ====== Pile-Up Effect ====== The Pile-Up effect describes the effects of photons lost at high photon count rates due... etect only one photon after one laser shot. "Pile up" describes the effects of photons lost at hight p
fluorescence_correlation_spectroscopy-_a_short_introduction @general
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es through the detection volume of a confocal set-up. Since then a series of studies have been perfor... se of point, single color FCS with a confocal set up will be examined. For FCS of freely diffusing fl... in FCS studies. Assume that we use the same set-up to measure the diffusion of three globular fluo... e "dark" in terms of fluorescence and long-lived (up to ~ms). Imaginge that a fluorophore is excited a
configuring_symphotime64_after_installation @support
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4 * to use SymPhoTime64 with a "home-made" set-up. ====Before Proceeding:==== These short instruct... hoTIme the user has to choose the exprimental set up type for which the SymPhoTime 64 will be used. \\... ce driver and press on "Connect". * You can set up the Laser Heads by pressing "Setup Heads" and a new window will pop up: {{ :support:laser_settings_setup_head.png?300 |
t3-mode @glossary
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. The main objective is to allow high sync rates (up to 84 MHz) which could not be handled in T2 Mode.... nted by four bits in each TTTR record, that allow up to four routing channels to be distinguished. How... ]] (First In First Out) buffer capable of holding up to 256 k event records. The FIFO input is fast en... the full speed of the time-to-digital converters (up to 10 Mcps each). This means, even during a fast
flim_fret_calculation_for_multi_exponential_donors @howto
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time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ates have been significantly higher than the pile up limit, or in cases, where the repetition rate has... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ET efficiency around 0 is expected and also shows up in the histogram. * On the lower right, the
separation_of_2_species_with @howto
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esponse:** The FLCS pattern matching windows pops up. {{ separation_of_2_species_with_Image_8.png?600 ... Image_9.png }} **Response:** Another window pops up containing the measurement files from the workspa... _Image_13.png }} **Response:** A new window pops up containing the measurement files from the workspa... g }} **Response:** The TCSPC fitting window pops up. {{ separation_of_2_species_with_Image_28.png?600
supported_mt200_pc_configuration_for_symphotime_32 @software
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indows * Check if the Peak CAN Controller Shows up in the Device Manager: {{ :support:device_manager... correctly installed (mvSigma, mVDelta should show up under Audio, Video and Gamecontroller): {{ :suppo... pported: TimeHarp 200, all versions PicoHarp 300, up to version HydraHarp 400, up to version 1.2 * NOT SUPPORTED: TimeHarp 260, HydraHarp 2.0 and higher
calculate_ratiometric_single_pair_fret_distributions_using_the_pie-fret_script @howto
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ript_Image_17.png }} **Response:** A window pops up asking for a file name and a place to store the u... your profile there. **Response:** A window pops up with the message that the software needs to be re... _Image_21.png?600 }} **Response:** A window pops up with the message that the software needs to be re
calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script @howto
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}} **Response:** The FCS Calibration window pops up. {{ calibrate_the_confocal_volume_for_fcs_using_t... ript_Image_21.png }} **Response:** A window pops up asking to confirm the calibration values. * Cli... rmed whenclosing the software. A message will pop up when closing SymPhoTime 64: {{ calibrate_the_con
how_to_measure_the_instrument_response_function_irf @howto
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es, provided that the system has had time to warm up (~15min), and neither the repetition rate nor the... found along the light pathway in a microscope set up. However, in case of cuvette based measurement ... ever, the dye must be pure, otherwise you may end up with measuring (long lifetime) impurity emission.
microtime @products
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75 nm to 810 nm * Multiple detector options for up to four detection channels * XY-scanning piezo ... imaging * Wide range scanner with a scan range up to several cm See the picoquant.com webpage for... r multicolor excitation from 375 nm to 900 nm * Up to 4 truly parallel detection channels using appl
differential_count_rate @glossary
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{{tag> pile-up dead_time IRF differential_countrate}} ====== Differential Count Rate ====== Ideally,... various counting losses.(E.g. to avoid the [[pile-up effect]]). However, for an IRF measurement the sa
t2-mode @glossary
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]] (First In First Out) buffer capable of holding up to 256 k event records. The FIFO input is fast en... the full speed of the time-to-digital converters (up to 10 Mcps each). This means, even during a fast
calculate_ratiometric_single_pair_fret_distributions @howto
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ions_Image_16.png }} **Response:** A window pops up asking for a file name and a place to store the u... _Image_19.png?339 }} **Response:** A window pops up with the message that the software needs to be re
lifetime-fitting_using_the_flim_script @howto
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lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
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update @howto
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using_the_anisotropy_image_script @howto
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using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs @howto
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visualizing_dynamics_with_the_multi_frame_flim_analysis @howto
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how_to_reinstall_symphotime
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some_origins_of_multiexponetial_decays_for_single_dyes
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novaflim_calculated_irf @faq
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the_dark_counts_of_my_spads_increased._when_do_i_need_to_take_action @faq
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dead_time @glossary
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antibunching_measurements @howto
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calculate_and_fit_fcs_traces_with_the_fcs_script @howto
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data_file_import @howto
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flim-fret_calculation_for_single_exponential_donors @howto
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intensity_time_trace_analysis @howto
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lifetime_fitting_using_the_flim_analysis @howto
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pattern_matching @howto
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roi_fitting_using_the_flim_script @howto
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symphotime_tips_and_tricks @howto
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visualizing_dynamics_using_the_multiframe-flim_script @howto
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syntax @wiki
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welcome @wiki
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old_dongles_drivers_for_fluofit_symphotime_32 @faq:old_dongles_drivers_for_fluofit_symphotime_32
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