time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ates have been significantly higher than the pile up limit, or in cases, where the repetition rate has... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ET efficiency around 0 is expected and also shows up in the histogram.
* On the lower right, the
es, provided that the system has had time to warm up (~15min), and neither the repetition rate nor the... found along the light pathway in a microscope set up.
However, in case of cuvette based measurement ... ever, the dye must be pure, otherwise you may end up with measuring (long lifetime) impurity emission.
background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... age was acquired are significantly above the Pile Up limit. It is also needed, if the pulse frequency