etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (up t... lifetime corresponds to the real average arrival time of the photons and is therefore also close to the... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f
te software suite for acquisition and analysis of time resolved fluorescence data. SymPhoTime features a...
EasyTau is the systems software of PicoQuants time resolved spectrometer [[products:FluoTime 300]].
... ss communication and configuration with PicoQuant Time Correlated Single Photon Counting Instruments (TC... nce-Tools/tttrlib|tttrlib]] to read and correlate time-tagged time resolved (TTTR) files.
* [[http:/
ved.
Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose.
=... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
0 MHz. Note the peak near the expected dip at lag-time=0 due to SPAD afterglow. Compared with figure 1 t... )
*//Delta// is the step width of the lag-time axis in units of the [[Products:PicoHarp 300]] Re... -Axis of the correlation.
*Start with lag-time/200/T2-resolution (e.g. 700ns/200/32ps ~=110; thi
1 Col 3 |
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^ Heading 5 | Row 2 Col... Col 3 |
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^ Heading 5 | Row 2 C... locks described in the previous section, but this time the name of the language syntax to be highlighted
etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... pulse does not shift within the image acquisition time within the TCSPC window, therefore we fix the par... e "Fast Lifetime", which is just the mean arrival time and contains e.g. background photons.\\
* An up
etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (up t... the decay in the TCSPC window. As a mean arrival time is calculated, it is possible that in dark region
etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... th a very high countrate and a system with a dead time significantly above the pile-up-limit or when the
opulation of freely diffusing molecules. In short time, the theoritical framework of FCS was expanded to... udy of rotation diffusion. Unfortunately, at that time the application of FCS was impractical due to the... e mean product of the fluorescence intensity F at time t with the fluuorescence intensity after a lag time τ divided by the mean fluorescence intensity square
ths of all contributions.
The observed decay in a time domain measurement is a convolution of the 'real'... pdated.\\
4. Select the decay range by Setting a `Time Gate` (move the red bars) to limit the decay the ... sed for the timegate.
* In order to define the time range better, double click on the graph with the ... indow with you can enlarge in order to define the time gates better. When content, click o.k.
* Deca
age containing many frames is treated here like a time-series. The example image shows the plasma membra... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... ame binning of 10 in order to decrease processing time and increase photon numbers per pixel, while stil
etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... exponentials that have a lifetime longer than the time constant of the systematic errors.
* Try last ... the "fast Lifetime", based on the photon arrival time only. The Histogram on the right of the image con
indow3.png |}}
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If necessary, change the time axis limits by dragging the appropriate vertical ... re dedicated sliders for both IRF and decay curve time ranges. Pressing the "Fit Single" button performs