g>tutorial howto analysis FLIM imaging FRET SymPhoTime}}
~~NOTOC~~
====== FLIM-FRET Calculation for M...
This tutorial shows step-by-step, how the "Lifetime FRET Image" analysis of SymPhoTime 64 can be used to calculate pixel-by-pixel the average [[glossary:fr... average FRET-efficiency, first the donor only lifetime is determined using cells that have only been tra
ial =====
==== Microscopy ====
[[software:SymPhoTime64]] \\
A complete software suite for acquisition and analysis of time resolved fluorescence data. SymPhoTime features a powerful scripting language that allows customisation... sis is currently not included in [[products:SymPhoTime64]]. However, you can use [[http://www.lfd.uci.ed
ved.
Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose.
=... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
{{tag>howto analysis lifetime tutorial SymPhoTime}}
~~NOTOC~~
====== SymPhoTime Lifetime Fitting ======
===== Summary =====
This tutorial shows step-by-step, how to fit a selecte
etectors slightly.
===== Antibunching using SymPhoTime =====
*with [[Products:PicoHarp 300]] in [[g... ossary:T3-mode]]
*not possible with SymPhoTime (32-bit version), because in SPT32 the Sync Divid... 400]] softwares as well as with [[software:SymPhoTime64]], where the sync divider can be disabled.
Usi... *measurements are possible with [[software:SymPhoTime64]]
==== Changing from Normal Acquisition (T3-mo
1 Col 3 |
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^ Heading 5 | Row 2 Col... Col 3 |
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^ Heading 5 | Row 2 C... locks described in the previous section, but this time the name of the language syntax to be highlighted
{{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ======
===== Introduction ====
g> howto FLIM imaging ROI analysis tutorial SymPhoTime}}
~~NOTOC~~
====== ROI Fitting Using the FLIM A... [[glossary:flim|FLIM]] script of [[products:SymPhoTime64|SymPhoTime 64]] can be used to fit several regions of interest (ROIs), and how to extract and interpr... start the script ====
* Start [[products:SymPhoTime64|SymPhoTime 64]] software.
* Open the "Samples
{{tag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)======
===== Introduction =====
This tutoria... shows step-by-step, how the FLIM script of SymPhoTime 64 can be used to fit several regions of interest... file and start the analysis ====
* Start SymPhoTime 64 software (this tutorial uses version 2.5, ther
{{tag>SymPhoTime MicroTime200 Scanner PI Physik_Instrumente E725 E710 MadCityLabs Mad_City_Labs PC}}
~~TOC~~
====== Su... ted MT200 PC and Hardware Configuration for SymPhoTime 64 ======
**NOTE:** When upgrading your system w... * WinXP: NO
=====Supported Hardware (for MicroTime200):=====
====Harps (TCSPC units)====
===PicoHa
{{tag>MicroTime LSM Theory SymPhoTime FCS Correlation}}
~~TOC~~
====== Introduction to Fluorescence Correlation Spect... opulation of freely diffusing molecules. In short time, the theoritical framework of FCS was expanded to... udy of rotation diffusion. Unfortunately, at that time the application of FCS was impractical due to the
{{tag>howto MicroTime200 dichroic microscopy}}
====== How to exchange the main dichroic of the MicroTime 200 ======
This video serves as additional supp... or the "Exchange of the main dichroic of the MicroTime 200." section of the MicroTime 200 manual. It is only valid for systems equipped with a 3mm dichroic.
{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}
====== How to Work with Instrument Response ... cts how a component with an infinitive short
lifetime is seen by the instrument. Of course, all compone... ths of all contributions.
The observed decay in a time domain measurement is a convolution of the 'real'... by-step analysis incl. A measured IRF using SymphoTime software: =====
1. First the measured IRF need