Table of Contents
Measurement Hardware / Instrumentation
Spectroscopy
Fluorescence Lifetime Measurements
Time-Resolved Fluorescence Anisotropy Measurements
Time Resolved Emission Spectra (TRES)
Measuring the Quantum Yield with the integrating Sphere assembly for the FT300
Microscopy
Quick Reference for Confocal Time-resolved Microscopy (FLIM, FRET, FCS) - Poster (pdf format)
Fluorescence and fluorescence microscopy by Nico Stuurman
Confocal microscoy and optical sectioning by Kurt Thorn
How to check the overlap of different color confocal volumes
Determination of the focal width with the focal width analysis
Two Photon Microscopy (TPM, TPE) by Kurt Thorn
Advantages and disadvantages of Two Photon Excitation (TPE)
How to measure the Instrument Response function (IRF)
FLIM Measurement using a Nikon A1 with a FLIM and FCS upgrade
Recording a Fluorescence Lifetime Image (FLIM) Stack with a LSM Upgrade Kit on a Nikon A1
FLIM-FRET Measurement using an Olympus FV1200 with a FLIM and FCS upgrade
Performing an FCS measurement with an Olympus FV1200 upgrade kit