In detail, this tutorial covers:
* Different file-types used or generated by SymPhoTime64
* Image... tional options and tools otherwise hidden
===== File-Type Overview =====
^Workspace: may contain these Filetypes ^ Content of the file ^ How to get such a file in the workspace ^ How to open/process^
|''.ptu''|Raw data in a unified TTTR-f
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... pie-fret_script_Image_1.png }}
* Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c... Response:** The PIE FRET script is applied to the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' and a new window op
}}
===== Step-by-Step Tutorial =====
Select a file and start the script
* Start [[products:SymPho... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... _fitting_script_Image_1.png }}
* Highlight the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mou... nse:** The TCSPC Fitting script is applied to the file ''ATTO655_diff_FLCS-pattern.ptu''. Thereby, a new
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... t_distributions_Image_1.png }}
* Highlight the file ''Cy3+Cy5_diff_PIE-FRET.ptu'' by a single mouse c...
**Response:** The FRET script is applied to the file ''Cy3+Cy5_diff_PIE-FRET.ptu''. Thereby, a new win
arge }}
===== Step-by-Step =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... the_flim_script_Image_1.png }}
* Highlight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse ...
**Response:** The FLIM script is applied to the file ''DaisyPollen_cells_FLIM.ptu''. Thereby, a new Wi
==== Data file import ====
Create a new workspace or open an existing one:
{{ :howto:et2_createopen.png | File menu }}
Then select Import Files. A new dialog opens, where the data file is selected:
{{ :howto:et2_fileimport.png | Selects the file to be imported }}
The file selected above is a s
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**\\
... _2_species_with_Image_1.png }}
* Highlight the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu'' by a single mou...
**Response:** The FLCS script is applied to the file ''ATTO655+Cy5_diff_FCS+FLCS.ptu''. Thereby, a new
4 software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**\\
... onential_donors_Image_1.png }}
* Highlight the file ''CENP-labelled _cells_for_FRET.ptu'' by a single... **Response:**\\
The FLIM script is applied to the file ''CENP-labelled_cells_for_FRET.ptu''. Thereby, a ... png }}
**Response:**\\
A window opens, where the file containing the IRF can be selected.
* Select th
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... mage_script_Image_1.png?217 }}
* Highlight the file ''Cy5_immo_FLIM+Pol-imaging''.
{{ using_the_aniso... :** The Anisotropy Image script is applied to the file ''Cy5_immo_FLIM+Pol-Imaging.ptu''. Thereby, a new
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... moval_via_flccs_Image_1.png }}
* Highlight the file ''IBA488+IBA547_unlinked_mix.ptu'' by a single mouse click.
**Note:** This file contains a FCS measurement of a FCCS negative con
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... calculate_fccs_Image_1.png }}
* Highlight the file ''IBA488+547_crosslinked.ptu'' by a single mouse click.
**Note:** This file contains a FCS measurement of a FCCS positive con
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime... on 2.5).
* Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\
**Resp... e left side of the main window.
* Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' ​by a singl... ponse:​**\\ ​
The FLIM analysis is applied to the file ''Hyperosmotic_Shock_MDCK_Cells.ptu''. Thereby, a
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File -> Open Workspace" from the main menu.
**Note:**... lizing_dynamics_Image_1.png }}
* Highlight the file ''GUVs.ptu'' by a single mouse click.
{{ visualiz... * The "Multi Frame FLIM" script is applied to the file ''GUs.ptu''. Thereby, a new Window opens:
{{ visu
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... me_trace_script_Image_1.png }}
* Highlight the file ''Cy5_immo_Lifetime_Trace.ptu'' by a single mouse click.
**Note:** This file contains a fluorescence time trace of an immobili
===== Step-by-Step Tutorial =====
==== Select a file and generate a reference FCS curve ====
* Star... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... ibration_script_Image_1.png }}
* Highlight the file ''ATTO4888_485nm_pulsed.ptu'' by a single mouse c... }
**Response:** The FCS script is applied to the file ''ATTO488_485nm_pulsed.ptu''. Thereby, a new wind