4 software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**\\
... onential_donors_Image_1.png }}
* Highlight the file ''CENP-labelled _cells_for_FRET.ptu'' by a single... **Response:**\\
The FLIM script is applied to the file ''CENP-labelled_cells_for_FRET.ptu''. Thereby, a ... png }}
**Response:**\\
A window opens, where the file containing the IRF can be selected.
* Select th
}}
===== Step-by-Step Tutorial =====
Select a file and start the script
* Start [[products:SymPho... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... _fitting_script_Image_1.png }}
* Highlight the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mou... nse:** The TCSPC Fitting script is applied to the file ''ATTO655_diff_FLCS-pattern.ptu''. Thereby, a new
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime ... rsions).
* Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\
**Resp... e left side of the main window.
* Highlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse cli... by clicking on "Start".
**Response:**\\
The file ''Kidney_Cells_FLIM.ptu'' is opened with the FLIM
arge }}
===== Step-by-Step =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... the_flim_script_Image_1.png }}
* Highlight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse ...
**Response:** The FLIM script is applied to the file ''DaisyPollen_cells_FLIM.ptu''. Thereby, a new Wi
===== Step-by-step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime... rsions).
* Open the “Tutorials” workspace via **File -> Open Workspace** from the main menu.\\
**Resp... e left side of the main window.
* Highlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse cli... ponse:**\\
The FLIM analysis is applied to the file ''Kidney_Cells_FLIM.ptu''. Thereby, a new Window
ng used in the analysis. Therefore, highlight the file with the measured IRF (single mouse click on the .ptu-file)\\ An example measured IRF can be found in Tutori... |}}
6. Click on “Export Decay as IRF”. Rename the file accordingly. The IRF can now be used for the fitt... laser settings (Please see `System Specification File` which is left by installation in your lab. The m
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime... on 2.5).
* Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\
**Resp... e left side of the main window.
* Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' by a singl... ponse:**\\
The FLIM analysis is applied to the file ''Hyperosmotic_Shock_MDCK_Cells.ptu''. Thereby, a
===== Step-by-Step Tutorial =====
==== Select a file and start the analysis ====
* Start SymPhoTime... on 2.5).
* Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.
**Respo... left side of the main window.
* Highlight the file ''MicroBeads.ptu'' by a single mouse click.
... nse:**\\
The FLIM analysis is applied to the data file. Thereby, a new Window opens:
{{ howto:sptwindow
of various timing electronics may use other data file formats. Possible file name extensions are ''.thd, .nhd, .phd'' and ''.hhd'' In order to analyze such f... existing EasyTau2 workspace.
* [[howto:data_file_import|Data file import]]
* [[howto:reconvolution_fit|Reconvolution fit]]
===== Samples =====
==== Data file import ====
Create a new workspace or open an existing one:
{{ :howto:et2_createopen.png | File menu }}
Then select Import Files. A new dialog opens, where the data file is selected:
{{ :howto:et2_fileimport.png | Selects the file to be imported }}
The file selected above is a s
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File -> Open Workspace" from the main menu.
**Note:**... lizing_dynamics_Image_1.png }}
* Highlight the file ''GUVs.ptu'' by a single mouse click.
{{ visualiz... * The "Multi Frame FLIM" script is applied to the file ''GUs.ptu''. Thereby, a new Window opens:
{{ visu
oTime 64]].
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:**
T... -fret-1expd_samples_ws.png |}}
* Highlight the file ''FRET_GFP and mRFP.ptu'' by a single mouse click... “Lifetime FRET Image” analysis is applied to the file ''FRET_GFP.ptu and mRFP.ptu''. Thereby, a new Win... al_donors_Image_15.png }}
**Response:** A result file is generated and linked to the raw data file.
{{
In detail, this tutorial covers:
* Different file-types used or generated by SymPhoTime64
* Image... tional options and tools otherwise hidden
===== File-Type Overview =====
^Workspace: may contain these Filetypes ^ Content of the file ^ How to get such a file in the workspace ^ How to open/process^
|''.ptu''|Raw data in a unified TTTR-f
===== Step-by-Step Tutorial =====
==== Select a file and start the script ====
* Start [[products:S... ] software.
* Open the "Samples" workspace via "File\open Workspace" from the main menu.
**Note:** Th... calculate_fccs_Image_1.png }}
* Highlight the file ''IBA488+547_crosslinked.ptu'' by a single mouse click.
**Note:** This file contains a FCS measurement of a FCCS positive con
mage|Structure of the pre-histogrammed Image Data File]]))
{{ :howto:bin_export_spt64.png?600 |}}
=====... 013.08.01%20-%20Reading%20the%20PicoQuant%20BIN%20file.pdf|Reading PicoQuant BIN files]] tutorial of the... hePICOQUANTfile.pdf| Function: Read the PicoQuant file ]] \\ \\
Users of SymPhoTime 32 with a PicoHarp