pplication note [[http://www.picoquant.com/images/uploads/page/
files/7267/appnote_flim_fret.pdf]].
Th... The window contains five different regions:\\
**Upper left:** Imaging analysis options.\\
**Upper center:** Fast FLIM image, displayed in false color s... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo
icoQuant takes no responsibility and provides no support. In case of problems please contact the respe... isting. Alternatively you can also use [[https://support.picoquant.com]] to contact PicoQuant support to submit your software project.
==== Data Analysi... orescence Spectroscopy and Imaging by research group Seidel, HHU]]: A collection of independent sotftw
{{tag>howto IRF acquisition video Nikon LSM_upgrade}}
~~TOC~~
====== How to Measure the Instrument R... es, provided that the system has had time to warm up (~15min), and neither the repetition rate nor the... for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the posit... found along the light pathway in a microscope set up.
However, in case of cuvette based measurement
the afterflash peak is characteristic for the setup.
To get rid of the afterflash peak, it has been... nm excitation, emission around 520 nm) could be suppressed by inserting one 536/40 bandpass filters i... ns).
*//Higher Delta values// will speed up the calculation but result in a coarser correlati... Antibunching single-photon emission and blinking suppression of CdSe/ZnS quantum dots//. In: ACS //Nan
==== Formatting Syntax ======
[[doku>DokuWiki]] supports some simple markup language, which tries to make the datafiles to be as readable as possible. Th... und:playground|playground]] page. The simpler markup is easily accessible via [[doku>toolbar|quickbutt... oo.
===== Basic Text Formatting =====
DokuWiki supports **bold**, //italic//, __underlined__ and ''m
o tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ======
====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo...
**Response:**\\
FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
e window contains five different regions:\\
- **Upper left:** imaging analysis options.\\
- **Upper center:** [[glossary:fast_flim|Fast FLIM]] image, ... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... n this case, the scale has to be adapted.\\
- **Upper right:** Fluorescence Lifetime Histogram. Here
ag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)======
===== Introductio... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo...
**Response:**\\
The FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
MadCityLabs Mad_City_Labs PC}}
~~TOC~~
====== Supported MT200 PC and Hardware Configuration for SymPhoTime 64 ======
**NOTE:** When upgrading your system we strongly encourage you to d... sted PC from PicoQuant. While we try our best to support custom PC setups we cannot guarantee that all the components will work together.
=====Supported
es through the detection volume of a confocal set-up.
Since then a series of studies have been perfor... living cells, including for example diffusion -coupled interactions like ligand binding, oligomerizat... se of point, single color FCS with a confocal set up will be examined. For FCS of freely diffusing fluorescent molecules in a confocal setup the origin of the intensity fluctuations, will be
ime 200 ======
This video serves as additional support for the "Exchange of the main dichroic of the... arge }}
If you are unsure about any of the steps please contact** http://www.picoquant.com/support**.
{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}
====== How to Work with Instrument Respons... 2) and Click”Calculate Decay”. The decay is then updated.\\
4. Select the decay range by Setting a `T
ulti-frame imaging analysis dynamics tutorial LSM_upgrade SymPhoTime}}
====== Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above) ======
=====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo