pplication note [[http://www.picoquant.com/images/uploads/page/
files/7267/appnote_flim_fret.pdf]].
Th... The window contains five different regions:\\
**Upper left:** Imaging analysis options.\\
**Upper center:** Fast FLIM image, displayed in false color s... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo
{{tag>howto IRF acquisition video Nikon LSM_upgrade}}
~~TOC~~
====== How to Measure the Instrument R... es, provided that the system has had time to warm up (~15min), and neither the repetition rate nor the... for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the posit... found along the light pathway in a microscope set up.
However, in case of cuvette based measurement
parentheses.
===== Sectioning =====
You can use up to five different levels of headlines to structur... d audio formats. To mitigate the problem, you can upload your file in different formats for maximum br... 4 video:
{{video.mp4|A funny video}}
When you upload a ''video.webm'' and ''video.ogv'' next to th... xgraph text thinbasic tsql twig typoscript unicon upc urbi uscript vala vbnet vb vbscript vedit verilo
o tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ======
====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo...
**Response:**\\
FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
e window contains five different regions:\\
- **Upper left:** imaging analysis options.\\
- **Upper center:** [[glossary:fast_flim|Fast FLIM]] image, ... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... n this case, the scale has to be adapted.\\
- **Upper right:** Fluorescence Lifetime Histogram. Here
ag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)======
===== Introductio... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo...
**Response:**\\
The FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
guration for SymPhoTime 64 ======
**NOTE:** When upgrading your system we strongly encourage you to d... TimeHarp 200
* HydraHarp v.1.0 -**__ Firmware upgrade to HydraHarp v2.xx necessary__** before usi
es through the detection volume of a confocal set-up.
Since then a series of studies have been perfor... se of point, single color FCS with a confocal set up will be examined. For FCS of freely diffusing fl... in FCS studies.
Assume that we use the same set-up to measure the diffusion of
three globular fluo... regarding:
* [[http://www.picoquant.com/images/uploads/page/files/7353/appnote_diffusioncoefficient
{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}
====== How to Work with Instrument Respons... 2) and Click”Calculate Decay”. The decay is then updated.\\
4. Select the decay range by Setting a `T
ulti-frame imaging analysis dynamics tutorial LSM_upgrade SymPhoTime}}
====== Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above) ======
=====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo
ag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade reconvolution fitting}}
====== Lifetime-Fi... rdware with count-rates way beyond classical pile up limit.
For the principle of rapidFLIM - recordi... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... |}}
**Response:**\\
FLIM Preview image will get updated. You may need to adapt the contrast again. O
the user may be required when you want to:
* upgrade SymPhoTime64
* change from SymPhoTime to ... 4
* to use SymPhoTime64 with a "home-made" set-up.
====Before Proceeding:====
These short instruct... hoTIme the user has to choose the exprimental set up type for which the SymPhoTime 64 will be used. \\... options depending on using the SPT64 with an LSM upgrade kit or a [[products:MicroTime]]
{{ :suppor