pplication note [[http://www.picoquant.com/images/uploads/page/
files/7267/appnote_flim_fret.pdf]].
Th... The window contains five different regions:\\
**Upper left:** Imaging analysis options.\\
**Upper center:** Fast FLIM image, displayed in false color s... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo
{{tag>howto IRF acquisition video Nikon LSM_upgrade}}
~~TOC~~
====== How to Measure the Instrument R... es, provided that the system has had time to warm up (~15min), and neither the repetition rate nor the... for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the posit... found along the light pathway in a microscope set up.
However, in case of cuvette based measurement
the afterflash peak is characteristic for the setup.
To get rid of the afterflash peak, it has been... nm excitation, emission around 520 nm) could be suppressed by inserting one 536/40 bandpass filters i... ns).
*//Higher Delta values// will speed up the calculation but result in a coarser correlati... Antibunching single-photon emission and blinking suppression of CdSe/ZnS quantum dots//. In: ACS //Nan
o tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ======
====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo...
**Response:**\\
FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
e window contains five different regions:\\
- **Upper left:** imaging analysis options.\\
- **Upper center:** [[glossary:fast_flim|Fast FLIM]] image, ... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... n this case, the scale has to be adapted.\\
- **Upper right:** Fluorescence Lifetime Histogram. Here
ag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)======
===== Introductio... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo...
**Response:**\\
The FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
ime 200 ======
This video serves as additional support for the "Exchange of the main dichroic of the... arge }}
If you are unsure about any of the steps please contact** http://www.picoquant.com/support**.
{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}
====== How to Work with Instrument Respons... 2) and Click”Calculate Decay”. The decay is then updated.\\
4. Select the decay range by Setting a `T
ulti-frame imaging analysis dynamics tutorial LSM_upgrade SymPhoTime}}
====== Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above) ======
=====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo
ag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade reconvolution fitting}}
====== Lifetime-Fi... rdware with count-rates way beyond classical pile up limit.
For the principle of rapidFLIM - recordi... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... |}}
**Response:**\\
FLIM Preview image will get updated. You may need to adapt the contrast again. O
n of a well aligned polarized laser beam from the upper surface of a clean, empty coverslip. (Glass-air interface; inverted microscope with UPlanSApo 60x, NA = 1.2, water immersion objective.)... to enter at the center of the objective entrance pupil, and the beam must be of course aligned with th
first histogram, the IRF in this particular case, updates the plot. Now, a right-click on ''crv[0]'' o... howto:et_fitwindow2.png |}}
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The plot is updated again. After these actions the fitting model