time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ates have been significantly higher than the pile up limit, or in cases, where the repetition rate has... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ET efficiency around 0 is expected and also shows up in the histogram.
* On the lower right, the
es, provided that the system has had time to warm up (~15min), and neither the repetition rate nor the... found along the light pathway in a microscope set up.
However, in case of cuvette based measurement ... ever, the dye must be pure, otherwise you may end up with measuring (long lifetime) impurity emission.
background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... age was acquired are significantly above the Pile Up limit. It is also needed, if the pulse frequency
background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... tem with a dead time significantly above the pile-up-limit or when the image has been acquired with a
es through the detection volume of a confocal set-up.
Since then a series of studies have been perfor... se of point, single color FCS with a confocal set up will be examined. For FCS of freely diffusing fl... in FCS studies.
Assume that we use the same set-up to measure the diffusion of
three globular fluo... e "dark" in terms of fluorescence and long-lived (up to ~ms). Imaginge that a fluorophore is excited a
rdware with count-rates way beyond classical pile up limit.
For the principle of rapidFLIM - recordi... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo
4
* to use SymPhoTime64 with a "home-made" set-up.
====Before Proceeding:====
These short instruct... hoTIme the user has to choose the exprimental set up type for which the SymPhoTime 64 will be used. \\... ce driver and press on "Connect".
* You can set up the Laser Heads by pressing "Setup Heads" and a new window will pop up:
{{ :support:laser_settings_setup_head.png?300 |
time of the dark background is usually very long (up to 1/2 of the [[glossary:TCSPC]] window), which m... ining all frames.
* Shift the parameter pannel up and enter a frame binning of 6 and a (pixel) binn