time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ates have been significantly higher than the pile up limit, or in cases, where the repetition rate has... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... ET efficiency around 0 is expected and also shows up in the histogram.
* On the lower right, the
orescence Spectroscopy and Imaging by research group Seidel, HHU]]: A collection of independent sotftw... rfrom the analysis workflow put forward by the group.Please note that a trial version is available.
... -for-Single-Molecule-Spectroscopy|Researchgate group Open Computational Tools for Single-Molecule Spec
es, provided that the system has had time to warm up (~15min), and neither the repetition rate nor the... found along the light pathway in a microscope set up.
However, in case of cuvette based measurement ... ever, the dye must be pure, otherwise you may end up with measuring (long lifetime) impurity emission.
the afterflash peak is characteristic for the setup.
To get rid of the afterflash peak, it has been... ns).
*//Higher Delta values// will speed up the calculation but result in a coarser correlati
=====
[[doku>DokuWiki]] supports some simple markup language, which tries to make the datafiles to be... und:playground|playground]] page. The simpler markup is easily accessible via [[doku>toolbar|quickbutt... ll these.
You can use <sub>subscript</sub> and <sup>superscript</sup>, too.
You can use <sub>subscript</sub> and <sup>superscript</sup>, too.
You can
background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... age was acquired are significantly above the Pile Up limit. It is also needed, if the pulse frequency
background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... tem with a dead time significantly above the pile-up-limit or when the image has been acquired with a
es through the detection volume of a confocal set-up.
Since then a series of studies have been perfor... se of point, single color FCS with a confocal set up will be examined. For FCS of freely diffusing fluorescent molecules in a confocal setup the origin of the intensity fluctuations, will be... in FCS studies.
Assume that we use the same set-up to measure the diffusion of
three globular fluo
rdware with count-rates way beyond classical pile up limit.
For the principle of rapidFLIM - recordi... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo
4
* to use SymPhoTime64 with a "home-made" set-up.
====Before Proceeding:====
These short instruct... hoTIme the user has to choose the exprimental set up type for which the SymPhoTime 64 will be used. \\... en SymPhoTIme is open go to Settings->Hardware Setup
* The Hardware window will open in which you ca... ce driver and press on "Connect".
* You can set up the Laser Heads by pressing "Setup Heads" and a n