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A complete software suite for acquisition and analysis of time resolved fluorescence data. SymPhoTime features a powerful scripting language that allows customisation of existing analysis and even implementation of novel analysis techniques.

Phasor Analysis Phasor Analysis is currently not included in SymPhoTime64. However, you can use Globals developed by the Laboratory for Fluorescence Dynamics to analyse FLIM data via the Phasor approach.


EasyTau is the systems software of PicoQuants time resolved spectrometer FluoTime 300. EasyTau features a scripting language to automate more complex experiments. Examples of EasyTau scripts can be found on github:

Decay curve analysis software

Open Source

Please note that these are external projects for which PicoQuant takes no responsibility and provides no support. In case of problems please contact the respective authors. Also note that such projects may not be maintained continuously. In particular, please check if they use current versions of file formats and/or programming libraries before you invest in larger development work based on such projects.

If you develop improved or new code, please consider contributing your code back to the community.

The compilation below is probably not complete. If you are the maintainer of a software that deals with TCSPC data please edit this page and add your software listing. Alternatively you can also use to contact PicoQuant support to submit your software project.

Data Analysis

  • A Python library to read PTU files
    From the authors: “The main purpose of the library is to compute g2 from the photon arrival times as fast as possible, as our files often go into multi GB sizes. With a good SSD we can parse >3GB/s. It can also compute intensity time traces and post select photons based on that.”
  • PAM is a software package for quantitative analysis of fluorescence microscopy and spectroscopy data, with a focus on experiments using pulsed interleaved excitation.
  • dspFRET: Matlab code for analyzing TTTR data from PicoQuant. Typical application is analysis of single pair FRET measurements with pulsed interleaved excitation (PIE-FRET).
  • FoCuS-point: FoCuS-point utilises advanced time-correlated single-photon counting (TCSPC) correlation algorithms along with time-gated filtering and innovative data visualisation. FoCuS Homepage and paper

Data Acquisition

Controlling PicoQuant Devices

Sepia II and Solea
TimeHarp 260
PicoHarp 300
HydraHarp 400


GitHub repositories mentioning PicoQuant and supporting the PicoQuant t3r data file format:

software.txt · Last modified: 2018/09/11 11:46 by admin