A complete software suite for acquisition and analysis of time resolved fluorescence data. SymPhoTime features a powerful scripting language that allows customisation of existing analysis and even implementation of novel analysis techniques.
Phasor Analysis is currently not included in SymPhoTime64. However, you can use Globals developed by the Laboratory for Fluorescence Dynamics to analyse FLIM data via the Phasor approach.
Please note that these are external projects for which PicoQuant takes no responsibility and provides no support. In case of problems please contact the respective authors. Also note that such projects may not be
maintained continuously. In particular, please check if they use current versions of file formats and/or programming libraries before you invest in larger development work based on such projects.
If you develop improved or new code, please consider contributing your code back to the community.
The compilation below is probably not complete. If you are the maintainer of a software that deals with TCSPC data please edit this page and add your software listing. Alternatively you can also use https://support.picoquant.com to contact PicoQuant support to submit your software project.
is a general-purpose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model functions, covering a wide range of applications in standard confocal FCS. In addition, it contains equations dealing with different excitation geometries like total internal reflection (TIR).
: open source software for automation of lifetime-based FRET analysis. This is a Microsoft Windows application originally developed in C#. The main goal of this application is to provide the FLIM community a user-friendly application.
.Fluorescence Decay and Anisotropy Analysis.
:Pycorrelate computes fast and accurate cross-correlation over arbitrary time lags. Cross-correlations can be calculated on “uniformly-sampled” signals or on “point-processes”, such as photon timestamps. Pycorrelate allows computing cross-correlation at log-spaced lags covering several orders of magnitude. This type of cross-correlation is commonly used in physics or biophysics for techniques such as fluorescence correlation spectroscopy (FCS) or dynamic light scattering (DLS).
: QuickFit 3 is our open-source data evaluation software for FCS and imagingFCS (imFCS) measurement. Actually QuickFit 3 itself is a project manager and all functionality is added as plugins. A set of tested plugins is supplied together with the software.
. FLIMfit is an open source package for rapid analysis of large FLIM datasets.
Extensible Time-tag Analyzer
. ETA, the extensible time tag analyzer, is an event driven programming language with graphical user interface for analyzing, plotting, and fitting of time tagged data.More info.
is a graphical application to support the use of a Time-Correlated Single Photon Counting system for application to Positron Annihilation Lifetime Spectroscopy. https://github.com/avancra/Pals3D
ImageJ data readers-
A Python library to read PTU files
From the authors: “The main purpose of the library is to compute g2
from the photon arrival times as fast as possible, as our files often go into multi GB
sizes. With a good SSD we can parse >3GB/s. It can also compute intensity time traces and post select photons based on that.”
: Matlab code for analyzing TTTR data from PicoQuant. Typical application is analysis of single pair FRET measurements with pulsed interleaved excitation (PIE-FRET).
: FoCuS-point utilises advanced time-correlated single-photon counting (TCSPC) correlation algorithms along with time-gated filtering and innovative data visualisation. FoCuS Homepage
Demos about Controlling PicoQuant Devices
Sepia II and Solea