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- avoid_pile_up_effect_in_flim_measurements
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- flim_fret_calculation_for_multi_exponential_donors
- pplication note [[http://www.picoquant.com/images/uploads/page/ files/7267/appnote_flim_fret.pdf]]. Th... The window contains five different regions:\\ **Upper left:** Imaging analysis options.\\ **Upper center:** Fast FLIM image, displayed in false color s... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo
- avoid_pile_up_effect_in_flim_measurements
- {{tag> howto FLIM acquisition pile-up TCSPC}} ~~TOC~~ ====== How to Avoid the Pile-up Effect in FLIM Measurements ====== For a definition of the pile-up effect see here: [[glossary:pile-up_effect|Pile-Up Effect]] To avoid [[glossary:pile-up_effect|pile-
- lifetime-fitting_using_the_flim_script
- o tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}} ====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ====== ====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... **Response:**\\ FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
- separation_of_2_species_with
- s <F1> to open the corresponding help page. - **Upper center/right:** Intensity time trace. The disp... esponse:** The FLCS pattern matching windows pops up. {{ separation_of_2_species_with_Image_8.png?600 ... ut the meaning of the different parameters. - **Upper center:** TCSPC histogram of the imported patt... n yet, this graph doesn't contain any data. - **Upper right:** TCPSC curve of the measured data. Low
- symphotime_tips_and_tricks
- post-acquisition analysis is performed - speeds up the processing for additional analysis. - This ... times) * Frames (especially important for LSM upgrade kits where in general several frames are rec... with the MT200 this is done automatically, in LSM upgrade systems, which are not remotely controlled b... d "Pattern Matching" the feature is made availble upon clicking on the **"Resolution estimation"** tab
- using_the_flcs_script_for_spectral_crosstalk_removal_via_flccs
- o the tutorial [[Calculate FCCS Trace with the Grouped FCS Script]]. **Note:** This way you can compa... s <F1> to open the corresponding help page. - **Upper center/right:** Intensity time trace. The disp... esponse:** The FLCS pattern matching windows pops up. {{ using_the_flcs_script_for_spectral_crosstalk_... ut the meaning of the different parameters. - **Upper center:** The graph for the imported patterns.
- lifetime_fitting_using_the_flim_analysis
- \ The window contains five different regions:\\ **Upper left:** imaging analysis options.\\ **Upper center:** Fast FLIM image, displayed in a false color ... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... e. In this case, the scale has to be adapted.\\ **Upper right:** the Fluorescence Lifetime Histogram.
- calibrate_the_confocal_volume_for_fcs_using_the_fcs_calibration_script
- icoQuant (see [[https://www.picoquant.com/images/uploads/page/files/7353/appnote_diffusioncoefficient... <F1> to open the corresponding help page.\\ - **Upper center/right:** Intensity time trace. The disp... d from calculating the FCS trace by adjusting the upper vertical red limit bars in the small upper trace.\\ - **Lower center/right:** FCS trace window.
- flim_measurement_using_a_nikon_a1_with_a_flim_and_fcs_upgrade
- {{tag> FLIM acquisition Nikon demo video LSM_upgrade howto}} ====== FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade ====== This tutorial shows the recording of FLIM images using an LSM upgrade kit, in this case a Nikon A1. In FLIM-measur... of the data acquisition are analogous in all LSM upgrade kits. The movie was recorded in 02/2014 and
- flim-fret_calculation_for_single_exponential_donors
- Technical note: [[http://www.picoquant.com/images/uploads/page/files/7350/appnote_flim_overview.pdf|Fl... window contains five different regions: - **Upper left: Imaging analysis options.** - **Upper center: [[glossary:fast flim|Fast FLIM]] image, disp... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo
- calculate_and_fit_fcs_traces_with_the_fcs_script
- om PicoQuant [see http://www.picoquant.com/images/uploads/page/files/7353/appnote_diffusioncoefficient... <F1> to open the corresponding help page.\\ - **Upper center/right:** the intensity time trace. The ... d from calculating the FCS trace by adjusting the upper vertical red limit bars in the small upper trace.\\ - **Lower center/right:** the FCS trace wind
- calculate_fccs_trace_with_the_grouped_fcs_script
- NOTOC~~ ====== Calculate FCCS Traces with the Grouped FCS Script ====== ===== Summary ===== This t... of a dye-labeled DNA-oligonucleotide using the Grouped FCS script. {{ youtube>0Ht_L3a53E0?large }} =... down menu: {{grey_button.png}} * Start the Grouped FCS script by clicking on "Start". {{ calculate_fccs_Image_4.png }} **Response:** The Grouped FCS script is applied to the file ''IBA488+547_
- calculate_ratiometric_fret-images
- pplication note [[http://www.picoquant.com/images/uploads/page/ files/7267/appnote_flim_fret.pdf]].\\ ... e window contains five different regions:\\ - **Upper left:** imaging calculation analysis options.\\ - **Upper center:** Fast FLIM image, displayed in false ... gned yet, this image area is still empty.\\ - **Upper right:** FRET image. As it has not been calcul
- how_to_measure_the_instrument_response_function_irf
- {{tag>howto IRF acquisition video Nikon LSM_upgrade}} ~~TOC~~ ====== How to Measure the Instrument R... es, provided that the system has had time to warm up (~15min), and neither the repetition rate nor the... for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the posit... found along the light pathway in a microscope set up. However, in case of cuvette based measurement
- intensity_time_trace_analysis
- plot consists of several parts:\\ In the **small upper panel**, the complete time trace is plotted as... he subsection of the trace marked in green in the upper trace for the two channels.\\ **On the right**... t intensity values. * To export data, e.g. the upper PCH histogram, place the mouse cursor in that ... :** PicoQuant photon counting boards can register up to four external marker signals. These marker sig