e file ''CENP-labelled _cells_for_FRET.ptu'' by a single mouse click.
{{ flim-fret-calculation_for_multi-e... green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red an estimation... ''CENP-labelled _cells_for_FRET.ptu'' again by a single mouse click.
{{ flim-fret-calculation_for_multi-e... can only be calculated if the FRET donor dye has single exponential decay kinetics, changing the fitting
and configuration with PicoQuant Time Correlated Single Photon Counting Instruments (TCSPC devices). It h... github.com/OpenSMFS|Open Tools for Solution-Based Single-Molecule Fluorescence Spectroscopy]]**
* **FR... : software for burst analysis of freely-diffusing single-molecule FRET (smFRET) measurements including μs-... https://github.com/tritemio/PyBroMo|simulator for single molecule FRET experiments of freely diffusing par
e lifetime of a measured sample. As an example, a single exponential reconvolution fit is used to determin... t the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mouse click.
{{ lifetime_fitting_using_the_tcpsc_... ".
**Response:** Both data sets are fitted with a single exponential tailfit model. The values of the last
ode]]
*Start Point Measurement
*For a single immobilized molecule antibunching trace you will ... u P., Ko H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/... B: //Ultrafast dynamics of protein collapse from single-molecule photon statistics//. In: //Proceedings o
ghlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... oexpo_roi0_kidney_3.png |}}
** Response:**\\
A single exponential reconvolution fit is performed. In th... * In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
ight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse click.
{{ roi_fitting_using_the_flim_script... green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red an estimation... \\
In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
ghlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... eparated regions with a similar lifetime into one single ROI, as it's done here, you have to press "Shift"... to:inner_monoexpo_3.png |}}
** Response:**\\
A single exponential reconvolution fit is performed. In th
==
In this short article only the case of point, single color FCS with a confocal set up will be examined... be monitored whereas in ~µs range processes like singlet-triplet kinetics, photo-isomerization and proton... anifested in the auto-correlation function is the singlet-triplet kinetics. Triplet states are "dark" in t... housands times, cycling between the first excited singlet state S1 and the ground state S0. With a certain
file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' by a single mouse click.
* Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... it_multiframe_2.png?300 |}}
** Response:**\\
A single exponential reconvolution fit is performed. In th... * In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
* Highlight the file ''MicroBeads.ptu'' by a single mouse click.
* Select the **"Analysis"** tab... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... { howto:monoexpo_roi0.png |}}
**Response:**\\
A single exponential fit is performed. In the TCSPC window... ll as the IRF background and IRF Fit.
* One the single pixel level, the background can be neglected, and
RF and decay curve time ranges. Pressing the "Fit Single" button performs a fully automated fit of a single exponential decay model:
{{ :howto:et_fitwindow4.png