e file ''CENP-labelled _cells_for_FRET.ptu'' by a single mouse click.
{{ flim-fret-calculation_for_multi-e... green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red an estimation... ''CENP-labelled _cells_for_FRET.ptu'' again by a single mouse click.
{{ flim-fret-calculation_for_multi-e... can only be calculated if the FRET donor dye has single exponential decay kinetics, changing the fitting
e lifetime of a measured sample. As an example, a single exponential reconvolution fit is used to determin... t the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mouse click.
{{ lifetime_fitting_using_the_tcpsc_... ".
**Response:** Both data sets are fitted with a single exponential tailfit model. The values of the last
ode]]
*Start Point Measurement
*For a single immobilized molecule antibunching trace you will ... u P., Ko H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/... B: //Ultrafast dynamics of protein collapse from single-molecule photon statistics//. In: //Proceedings o
ghlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... oexpo_roi0_kidney_3.png |}}
** Response:**\\
A single exponential reconvolution fit is performed. In th... * In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
ight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse click.
{{ roi_fitting_using_the_flim_script... green (= dataset 0) and the TCSPC histogram of a single pixel in grey (= dataset 1). In red an estimation... \\
In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
ghlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse click.
* Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... eparated regions with a similar lifetime into one single ROI, as it's done here, you have to press "Shift"... to:inner_monoexpo_3.png |}}
** Response:**\\
A single exponential reconvolution fit is performed. In th
file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' by a single mouse click.
* Select the **"Analysis"** tab ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... it_multiframe_2.png?300 |}}
** Response:**\\
A single exponential reconvolution fit is performed. In th... * In this example it is obvious that the applied single exponential reconvolution fit is not suited to fi
* Highlight the file ''MicroBeads.ptu'' by a single mouse click.
* Select the **"Analysis"** tab... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation... { howto:monoexpo_roi0.png |}}
**Response:**\\
A single exponential fit is performed. In the TCSPC window... ll as the IRF background and IRF Fit.
* One the single pixel level, the background can be neglected, and
RF and decay curve time ranges. Pressing the "Fit Single" button performs a fully automated fit of a single exponential decay model:
{{ :howto:et_fitwindow4.png
.png }}
* Highlight the file ''GUVs.ptu'' by a single mouse click.
{{ visualizing_dynamics_Image_2.png ... aset 0) and the [[glossary:TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation
e}}
~~NOTOC~~
====== FLIM-FRET Calculation for Single Exponential Donors ======
===== Summary =====
... acceptor pair.
If the FRET donor molecule has a single exponential decay kinetic, not only the FRET effi... fret|FRET]].
Several fluorescent proteins with a single exponential decay behavior have been reported, e.... Highlight the file ''FRET_GFP and mRFP.ptu'' by a single mouse click.
{{ :howto:flim-fret-1expd_gret_gfp_a
e [[products:MicroTime]], the image consists of a single frame)
=== FCS (for each of the max.2 data chan... cay is chosen as 0. Background pixels with just a single photon which might be before this pixel can there...
* Every pixel in the image is exported as one single pixel in the bitmap
* The color scale is not e...
* Every pixel in the image is exported as one single pixel in the tiff
* the tiff format for the nu
ight the file ''IBA488+547_crosslinked.ptu'' by a single mouse click.
**Note:** This file contains a FCS ... lculate_fccs_Image_7.png }}
* Press "Calculate Single".
{{ calculate_fccs_Image_8.png }}
**Response:*... negative calibration probe, i.e. a mixture of two single labeled compounds needs to be measured. To demons