Performing an FCS measurement with a Olympus FV1200 upgrade kit

This is an old revision of the document!

howto, FCS, acquisition, video, FV1200, Olympus, LSM upgrade

This video is a walkthrough of how to measure a FCS (Fluorescence Correlation Spectroscopy) trace with an Olympus FV1200 upgraded for FLIM and FCS with a PicoQuant upgrade kit.

Continue to:

Calculate and Fit FCS Traces with the FCS Script
Calculate FCCS Traces with the Grouped FCS Script
Calibrate the Confocal Volume for and with FCS
Separation of 2 Species with Different Lifetimes Using FLCS
Spectral Crosstalk Removal via FLCCS