======MT200 FCS Measurement ======
{{tag>howto MicroTime200 video tutorial}}
fluorescence correlation spectroscopy (FCS) is a quantitative measurement of the resolution
the script with the stored result
- For online FCS-results, the calculated molecular brightness can ... result-file opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on ... me]], the image consists of a single frame)
=== FCS (for each of the max.2 data channels) ===
<WRAP>
<imgcaption CommentFCS| Comment Tab of an FCS online analysis result>{{onlinefcs-3.png?300|}}</
{{tag> analysis howto tutorial FCS FCCS Cross-Correlation SymPhoTime}}
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====== Calculate FCCS Traces with the Grouped FCS Script ======
===== Summary =====
This tutoria... dye-labeled DNA-oligonucleotide using the Grouped FCS script.
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===== B... ngle mouse click.
**Note:** This file contains a FCS measurement of a FCCS positive control, a DNA oli
9%20-%20Reading%20PicoQuant%20PT3%20files%20for%20FCS%20and%20lifetime%20FCS.pdf| Reading PicoQuant PT3 files for FCS and lifetime FCS]]
Additional Tutorials for the Globals software can be found here: [[http://www.lfd
wto analysis calibration PSF confocal-volume VEFF FCS SymPhoTime}}
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====== Calibrate the Confocal Volume for and with FCS ======
===== Summary =====
This tutorial shows... by-step, how to calibrate the confocal volume for FCS measurements with a calibration dye. Therefore, a FCS curve is calculated with the FCS script, that is
{{tag> 2fFCS FCS}}
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====== 2fFCS ======
2fFCS is an abbreviation **2 F**ocus **F**luorescence **C**orrelation **S**pectroscopy. Dual focus FCS might also be used.
Correlation analysis that is... fusion length into the analysis.
see [[glossary:FCS]] for a introduction on FCS.
===== Experimental Setup =====
==== Pinhole Selection ====
The conjugat
{{tag> tutorial howto FCS analysis SymPhoTime}}
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====== Calculate and Fit FCS Traces with the FCS Script ======
===== Summary =====
This tutorial shows step-by-step, how an auto... olved in water is calculated and fitted using the FCS script.
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===== B
FLIM Measurement Using a Nikon A1 with a FLIM and FCS Upgrade ======
This tutorial shows the recording... NIS Elements 4.3 integrates control for FLIM and FCS =====
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The movie... Kit on a Nikon A1 ]]
* [[FLIM-FRET Measurement using an Olympus FV1200 with a FLIM and FCS upgrade]]
{{tag> howto tutorial FCS FLCS crosstalk analysis SymPhoTime}}
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====== Spectral Crosstalk Rem... tutorial [[Calculate FCCS Trace with the Grouped FCS Script]].
**Note:** This way you can compare the... ngle mouse click.
**Note:** This file contains a FCS measurement of a FCCS negative control (two DNA o... lysis" tab and in there, open the drop down menu "FCS".
{{ using_the_flcs_script_for_spectral_crosstalk
{{tag> howto FCS acquisition video FV1200 Olympus LSM_upgrade}}
====== Performing a FCS measurement with an Olympus FV1200 Upgrade Kit===... }
This video is a walkthrough of how to measure a FCS (Fluorescence Correlation Spectroscopy) trace with an Olympus FV1200 upgraded for FLIM and FCS with a PicoQuant upgrade kit.
Continue to:
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