erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (up t... lifetime corresponds to the real average arrival time of the photons and is therefore also close to the
ved.
Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose.
=... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
0 MHz. Note the peak near the expected dip at lag-time=0 due to SPAD afterglow. Compared with figure 1 t... )
*//Delta// is the step width of the lag-time axis in units of the [[Products:PicoHarp 300]] Re... -Axis of the correlation.
*Start with lag-time/200/T2-resolution (e.g. 700ns/200/32ps ~=110; thi
erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... pulse does not shift within the image acquisition time within the TCSPC window, therefore we fix the par
erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (up t... the decay in the TCSPC window. As a mean arrival time is calculated, it is possible that in dark region
erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... th a very high countrate and a system with a dead time significantly above the pile-up-limit or when the
ths of all contributions.
The observed decay in a time domain measurement is a convolution of the 'real'... see this kind of curve:
{{ howto:irf_decay_before_timegate.png?800 |}}
(Before selecting a detection cha... pdated.\\
4. Select the decay range by Setting a `Time Gate` (move the red bars) to limit the decay the ...
**Note:**
* The green area is used for the timegate.
* In order to define the time range bette
age containing many frames is treated here like a time-series. The example image shows the plasma membra... erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with
erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... ad over the TCSPC window, the mean photon arrival time of the dark background is usually very long (with... exponentials that have a lifetime longer than the time constant of the systematic errors.
* Try last
indow3.png |}}
\\
\\
If necessary, change the time axis limits by dragging the appropriate vertical ... re dedicated sliders for both IRF and decay curve time ranges. Pressing the "Fit Single" button performs
s://www.picoquant.com/products/category/tcspc-and-time-tagging-modules|TimeHarp260, PicoHarp300, HydraHarp400 or MultiHarp150]] is controlled via EasyTau 2. S... ad of control via EasyTau 2. Recent versions of ''TimeHarp260.exe'', ''PicoHarp.exe'', ''HydraHarp.exe''
on of the [[https://www.picoquant.com/dl_software/TimeHarp260/TimeHarp260_SW_and_DLL_V3_2.zip|native TimeHarp260 software]]. This file contains two histograms: an [[howto
st arriving photon against a photon at the longer time, which will be an increasingly unlikely event bec... experiment restarts.
A simulation with 90ns dead-time period:
[{{howto:logf.png?320x240|Fig. 2: Simulation }}]
\\
The timescale is in ns. The plateau is the [[glossary:dead time]]. The decay slope depends on the count rate.
Th
erage "Fast FLIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not d... etch from minimum to maximum. As the mean arrival time of the background photons in the areas where no f... [glossary:TCSPC]] window, the mean photon arrival time of the dark background is usually very long (up t... and a (pixel) binning of 3 to decrease processing time and increase photon numbers per pixel.
{{ visuali