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- lar, please check if they use current versions of file formats and/or programming libraries before you i... s, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in mod... ttps://github.com/PicoQuant/PicoQuant-Time-Tagged-File-Format-Demos| Demo Code for reading PicoQuants PTU File format]] * [[https://github.com/Brinkslab/ptu_
- syntax @wiki
- is behavior can be enabled in the [[doku>config]] file. Hint: If DokuWiki is a link, then it's enabled. ... ?php /** * Customization of the english language file * Copy only the strings that needs to be modifie... ats. To mitigate the problem, you can upload your file in different formats for maximum browser compatib... filename as the video and be either a jpg or png file. In the example above a ''video.jpg'' file would
- howto @howto
- of various timing electronics may use other data file formats. Possible file name extensions are ''.thd, .nhd, .phd'' and ''.hhd'' In order to analyze such f... existing EasyTau2 workspace. * [[howto:data_file_import|Data file import]] * [[howto:reconvolution_fit|Reconvolution fit]] ===== Samples ==
- flim_fret_calculation_for_multi_exponential_donors @howto
- 4 software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:**\\ ... onential_donors_Image_1.png }} * Highlight the file ''CENP-labelled _cells_for_FRET.ptu'' by a single... **Response:**\\ The FLIM script is applied to the file ''CENP-labelled_cells_for_FRET.ptu''. Thereby, a ... png }} **Response:**\\ A window opens, where the file containing the IRF can be selected. * Select th
- pre-histogrammed_image @glossary
- ==== Structure of the pre-histogrammed Image Data File====== ^Data Item ^^^^Type ^De... | |The following block will appear in the file for each ''y = 1 to <PixY>'' |||... an example in Python and Matlab see here: https://github.com/PicoQuant/pre-histogrammed-Image-Data-File
- lifetime_fitting_using_the_tcpsc_fitting_script @howto
- }} ===== Step-by-Step Tutorial ===== Select a file and start the script * Start [[products:SymPho... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... _fitting_script_Image_1.png }} * Highlight the file ''ATTO655_diff_FLCS-pattern.ptu'' by a single mou... nse:** The TCSPC Fitting script is applied to the file ''ATTO655_diff_FLCS-pattern.ptu''. Thereby, a new
- lifetime-fitting_using_the_flim_script @howto
- ===== Step-by-step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime ... rsions). * Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\ **Resp... e left side of the main window. * Highlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse cli... by clicking on "Start". **Response:**\\ The file ''Kidney_Cells_FLIM.ptu'' is opened with the FLIM
- roi_fitting_using_the_flim_script @howto
- arge }} ===== Step-by-Step ===== ==== Select a file and start the script ==== * Start [[products:S... ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. **Note:** Th... the_flim_script_Image_1.png }} * Highlight the file ''DaisyPollen_cells_FLIM.ptu'' by a single mouse ... **Response:** The FLIM script is applied to the file ''DaisyPollen_cells_FLIM.ptu''. Thereby, a new Wi
- update @howto
- ===== Step-by-step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime... rsions). * Open the “Tutorials” workspace via **File -> Open Workspace** from the main menu.\\ **Resp... e left side of the main window. * Highlight the file ''Kidney_Cells_FLIM.ptu'' by a single mouse cli... ponse:**\\ The FLIM analysis is applied to the file ''Kidney_Cells_FLIM.ptu''. Thereby, a new Window
- how_to_work_with_the_instrument_response_function_irf @howto
- ng used in the analysis. Therefore, highlight the file with the measured IRF (single mouse click on the .ptu-file)\\ An example measured IRF can be found in Tutori... |}} 6. Click on “Export Decay as IRF”. Rename the file accordingly. The IRF can now be used for the fitt... laser settings (Please see `System Specification File` which is left by installation in your lab. The m
- visualizing_dynamics_using_the_multiframe-flim_script @howto
- ===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime... on 2.5). * Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu.\\ **Resp... e left side of the main window. * Highlight the file ''Hyperosmotic_Shock_MDCK_Cells.ptu'' by a singl... ponse:**\\ The FLIM analysis is applied to the file ''Hyperosmotic_Shock_MDCK_Cells.ptu''. Thereby, a
- lifetime-fitting_using_the_rapid_reconvolution_algorithm @howto
- ===== Step-by-Step Tutorial ===== ==== Select a file and start the analysis ==== * Start SymPhoTime... on 2.5). * Open the "Tutorials" workspace via **File -> Open Workspace** from the main menu. **Respo... left side of the main window. * Highlight the file ''MicroBeads.ptu'' by a single mouse click. ... nse:**\\ The FLIM analysis is applied to the data file. Thereby, a new Window opens: {{ howto:sptwindow
- configuring_symphotime64_after_installation @support
- Settings-> Save User Configuration. * Save the file to a folder so that you can have it as a back-up ... . You can create a new workspace or load goint to File→… {{ :support:workspace.jpg?200 |}} ====Bright/D
- data_file_import @howto
- ==== Data file import ==== Create a new workspace or open an existing one: {{ :howto:et2_createopen.png | File menu }} Then select Import Files. A new dialog opens, where the data file is selected: {{ :howto:et2_fileimport.png | Selects the file to be imported }} The file selected above is a s
- create_time_gated_image
- ] software. * Open the "Samples" workspace via "File\open Workspace" from the main menu. * Open the DaisyPollen-sample file with the FLIM-analysis * In the controls, you'l