pplication note [[http://www.picoquant.com/images/uploads/page/
files/7267/appnote_flim_fret.pdf]].
Th... The window contains five different regions:\\
**Upper left:** Imaging analysis options.\\
**Upper center:** Fast FLIM image, displayed in false color s... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo
{{tag>howto IRF acquisition video Nikon LSM_upgrade}}
~~TOC~~
====== How to Measure the Instrument R... es, provided that the system has had time to warm up (~15min), and neither the repetition rate nor the... for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the posit... found along the light pathway in a microscope set up.
However, in case of cuvette based measurement
o tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ======
====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo...
**Response:**\\
FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
e window contains five different regions:\\
- **Upper left:** imaging analysis options.\\
- **Upper center:** [[glossary:fast_flim|Fast FLIM]] image, ... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo... n this case, the scale has to be adapted.\\
- **Upper right:** Fluorescence Lifetime Histogram. Here
ag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade}}
====== ROI Fitting Using the FLIM Analysis (updated for SymPhoTime v2.5)======
===== Introductio... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo...
**Response:**\\
The FLIM Preview image will get updated. Optimize contrast by adapting "Min" and "
{{tag>howto IRF tutorial analysis MicroTime LSM_upgrade}}
====== How to Work with Instrument Respons... 2) and Click”Calculate Decay”. The decay is then updated.\\
4. Select the decay range by Setting a `T
ulti-frame imaging analysis dynamics tutorial LSM_upgrade SymPhoTime}}
====== Visualizing Dynamics Using the Multi Frame FLIM Analysis (updated for SymPhoTime v2.5 and above) ======
=====... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo
ag>howto tutorial FLIM analysis SymPhoTime MT LSM_upgrade reconvolution fitting}}
====== Lifetime-Fi... rdware with count-rates way beyond classical pile up limit.
For the principle of rapidFLIM - recordi... background is usually very long (with an average up to 1/2 of the TCSPC window), which makes the colo... |}}
**Response:**\\
FLIM Preview image will get updated. You may need to adapt the contrast again. O
n of a well aligned polarized laser beam from the upper surface of a clean, empty coverslip. (Glass-air interface; inverted microscope with UPlanSApo 60x, NA = 1.2, water immersion objective.)
first histogram, the IRF in this particular case, updates the plot. Now, a right-click on ''crv[0]'' o... howto:et_fitwindow2.png |}}
\\
\\
The plot is updated again. After these actions the fitting model
IM FRET acquisition howto demo Olympus FV1200 LSM_upgrade}}
====== FLIM-FRET Measurement Using an Olympus FV1200 with a FLIM and FCS Upgrade ======
This tutorial shows the recording of FLIM-FRET images using an LSM upgrade kit, in this case connected to an Olympus Fl... of the data acquisition are analogous in all LSM upgrade kits.
The movie was recorded in 02/2018; the
e window contains five different regions:\\
- **Upper left:** Imaging analysis options. As there are... bar is generated to access all controls.\\
- **Upper center:** [[glossary:Fast FLIM]] image display... time of the dark background is usually very long (up to 1/2 of the [[glossary:TCSPC]] window), which m... n this case, the scale has to be adapted.\\
- **Upper right:** Fluorescence Lifetime Histogram. Here
Technical note: [[http://www.picoquant.com/images/uploads/page/files/7350/appnote_flim_overview.pdf|Fl... window contains five different regions:
- **Upper left: Imaging analysis options.**
- **Upper center: [[glossary:fast flim|Fast FLIM]] image, disp... time of the dark background is usually very long (up to 1/2 of the TCSPC window), which makes the colo