ere might be slight differences compared to other software versions. For example the way of setting an inten... the FLIM analysis ====
* Start SymPhoTime 64 software.
* Open the "Samples" workspace via "File\open ... iexpd_reconvfit_response.png |}}
**Note:**\\
The software offers the possibility to fit the data using a n-... o:flim-fret-multiexpd_initialfit.png |}}
* The software calculates the amplitude weighted average lifetim
{{tag> software acquisition analysis}}
~~TOC~~
====== Software ======
===== Commercial =====
==== Microscopy ====
[[software:SymPhoTime64]] \\
A complete software suite for acquisition and analysis of time resolved fluorescence dat
* Start [[products:SymPhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open ... _fitting_script_Image_6.png?600 }}
**Note:** The software offers the possibility to use a n-exponential tai
roducts:PicoHarp 300]]/[[Products:HydraHarp 400]] softwares as well as with [[software:SymPhoTime64]], where the sync divider can be disabled.
Using a PicoHarp300, c... -mode]]
*measurements are possible with [[software:SymPhoTime64]]
==== Changing from Normal Acquisi... ver, it's enough to disable the SYNC in the SEPIA software => no cable change necessary.
=== Software Setti
d start the analysis ====
* Start SymPhoTime 64 software version (version 2.5 is used for this tutorial, t... n the fitting parameter table.\\
**Note:**\\
The software offers the possibility to fit the data using a n-
analysis|Lifetime fitting Using the FLIM analysis software]]
[[howto:roi_fitting_using_the_flim_script|ROI fitting using FLIM analysis software]]
[[howto:flim-fret_calculation_for_single_expon
* Start [[products:SymPhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open ... he_flim_script_Image_17.png?600 }}
**Note:** The software offers the possibility to fit the data using a n-
start the analysis ====
* Start SymPhoTime 64 software (this tutorial uses version 2.5, there might be d... { howto:addroi.png?400 |}}
**Response:**\\
The software assigns sequential numbers to selected ROIs.The w... n the fitting parameter table.\\
**Note:**\\
The software offers the possibility to fit the data using an n
Ie Framegrabber \\ [[https://www.picoquant.com/dl_software/MT200_Driver/mvDELTA_mvSIGMA-x86_64-2.18.3.zip]] ... k CAN PCIe board \\ [[http://www.picoquant.com/dl_software/MT200_Driver/PEAK_CAN_PCI.zip]] \\ Note: older ve
tion and fitting FCS curves with the SymPhoTIme64 software]]
* [[howto:calibrate_the_confocal_volume_for_f... ion of the confocal volume using the SymPhoTime64 software]]
The generalised form of equation $\ref{eq2}$ ... ude values larger than 1.
Nowadays hardware and software correlators are available which take the fluoresc
SEPIA laser driver or in the setting of the (LSM) software. Changing the electrical power of the laser chang... ep analysis incl. A measured IRF using SymphoTime software: =====
1. First the measured IRF needs to be m
start the analysis ====
* Start SymPhoTime 64 software (this tutorial uses version 2.5).
* Open the "T... n the fitting parameter table.\\
**Note:**\\
The software offers the possibility to fit the data using a n-... files, which can be opened e..g using open source software like Fiji\\
- BIN exports the image series as a... ge size information automatically via a handshake software, as in several LSMs, the image size is automatica
start the analysis ====
* Start SymPhoTime 64 software (This tutorial uses version 2.5).
* Open the "T... select an additional ROI, click on "New" ROI. The software gives sequential order numbers to your ROIs, so h
{{tag> SymPhoTime software howto}}
~~TOC~~
====== Configuring SymPhoTime64 ======
====When do you need to ... tion.__
Then you could use the Remote Controller software by installing it to the LSM computer. [[http://www.picoquant.com/dl_software/SPT-RC/SymPhoTime_RC_Setup.zip| SymPhoTime 64 Rem