ere might be slight differences compared to other software versions. For example the way of setting an inten... the FLIM analysis ====
* Start SymPhoTime 64 software.
* Open the "Samples" workspace via "File\open ... iexpd_reconvfit_response.png |}}
**Note:**\\
The software offers the possibility to fit the data using a n-... o:flim-fret-multiexpd_initialfit.png |}}
* The software calculates the amplitude weighted average lifetim
* Start [[products:SymPhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open ... _fitting_script_Image_6.png?600 }}
**Note:** The software offers the possibility to use a n-exponential tai
roducts:PicoHarp 300]]/[[Products:HydraHarp 400]] softwares as well as with [[software:SymPhoTime64]], where the sync divider can be disabled.
Using a PicoHarp300, c... -mode]]
*measurements are possible with [[software:SymPhoTime64]]
==== Changing from Normal Acquisi... ver, it's enough to disable the SYNC in the SEPIA software => no cable change necessary.
=== Software Setti
d start the analysis ====
* Start SymPhoTime 64 software version (version 2.5 is used for this tutorial, t... n the fitting parameter table.\\
**Note:**\\
The software offers the possibility to fit the data using a n-
* Start [[products:SymPhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File\open ... he_flim_script_Image_17.png?600 }}
**Note:** The software offers the possibility to fit the data using a n-
start the analysis ====
* Start SymPhoTime 64 software (this tutorial uses version 2.5, there might be d... { howto:addroi.png?400 |}}
**Response:**\\
The software assigns sequential numbers to selected ROIs.The w... n the fitting parameter table.\\
**Note:**\\
The software offers the possibility to fit the data using an n
SEPIA laser driver or in the setting of the (LSM) software. Changing the electrical power of the laser chang... ep analysis incl. A measured IRF using SymphoTime software: =====
1. First the measured IRF needs to be m
start the analysis ====
* Start SymPhoTime 64 software (this tutorial uses version 2.5).
* Open the "T... n the fitting parameter table.\\
**Note:**\\
The software offers the possibility to fit the data using a n-... files, which can be opened e..g using open source software like Fiji\\
- BIN exports the image series as a... ge size information automatically via a handshake software, as in several LSMs, the image size is automatica
start the analysis ====
* Start SymPhoTime 64 software (This tutorial uses version 2.5).
* Open the "T... select an additional ROI, click on "New" ROI. The software gives sequential order numbers to your ROIs, so h
kspace downloadable [[http://www.picoquant.com/dl_software/SPT64/Demo_workspace_SPT64.zip|here]] (1.5GB).
=... /fluorescence-spectrometers/easytau-2|EasyTau 2]] software are directly stored in a workspace in ''.etc'' da... utton.
* It is also possible to use the native software of the timing electronics for data acquisition, i... ore the histograms in ''.phu'' data format. Older software versions of various timing electronics may use ot
nstallation of the [[https://www.picoquant.com/dl_software/TimeHarp260/TimeHarp260_SW_and_DLL_V3_2.zip|native TimeHarp260 software]]. This file contains two histograms: an [[howto:... g.
The same data file, when opened in the native software, looks like this:
{{ :howto:et2_originalhistogra
* Start [[products:SymPhoTime64|SymPhoTime 64]] software.
* Open the "Samples" workspace via "File -> Op... ed with a scanner controlled by the SymPhoTime 64 software (i.e. the piezo scanner in the [[products:MicroTi
ere might be slight differences compared to other software versions. For example the way of setting an inten... ing the main menu ("Settings\Save User Profile as"). The software will need to restart to adapt the changes.