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video_tutorials [2014/06/18 11:58]
admin
video_tutorials [2014/11/17 17:36]
admin
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 ==== Confocal Microscopy ==== ==== Confocal Microscopy ====
 +Mark Armitage explains major components of confocal microscopes and their use.
  
-{{ youtube>PLO0yDSkagDljzI0bdM5m1njrdLdVa80_N?large }}+{{ youtube>BUSZD4IVYSw?large }} 
 +This video is part of a series of videos. This is the related playlist: [[https://​www.youtube.com/​playlist?​list=PLO0yDSkagDljzI0bdM5m1njrdLdVa80_N]]
  
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 Nikons website [[http://​www.microscopyu.com/​articles/​confocal/​index.html|MicroscopyU.com]] has a very nice course on confocal microscopy. Nikons website [[http://​www.microscopyu.com/​articles/​confocal/​index.html|MicroscopyU.com]] has a very nice course on confocal microscopy.
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 [[http://​www.ibiology.org/​ibioseminars/​techniques/​kurt-thorn-part-1.html]] [[http://​www.ibiology.org/​ibioseminars/​techniques/​kurt-thorn-part-1.html]]
  
-=== Kurt Thorn: Two Photon Microscopy ===+==== Kurt Thorn: Two Photon Microscopy ​====
 Kurt Thorn of UCSF talks about two-photon microscopy which uses intense pulsed lasers to image deep into biological samples. It can be used for imaging thick tissue specimens or even imaging inside of live animals. Kurt Thorn of UCSF talks about two-photon microscopy which uses intense pulsed lasers to image deep into biological samples. It can be used for imaging thick tissue specimens or even imaging inside of live animals.
  
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-=== Philippe Bastiaens: Fluorescence Lifetime Microscopy (FLIM) ===+==== Philippe Bastiaens: Fluorescence Lifetime Microscopy (FLIM) ​====
 Fluorescence-lifetime imaging microscopy or [[glossary:​flim|FLIM]] is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography. Fluorescence-lifetime imaging microscopy or [[glossary:​flim|FLIM]] is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography.
 The lifetime of the fluorophore signal, rather than its intensity, is used to create the image in FLIM. This has the advantage of minimizing the effect of photon scattering in thick layers of sample ([[wp>​Fluorescence-lifetime_imaging_microscopy | FLIM]]). The lifetime of the fluorophore signal, rather than its intensity, is used to create the image in FLIM. This has the advantage of minimizing the effect of photon scattering in thick layers of sample ([[wp>​Fluorescence-lifetime_imaging_microscopy | FLIM]]).
video_tutorials.txt · Last modified: 2014/11/17 17:36 by admin