{{tag> tutorial howto time-trace analysis SymPhoTime}}
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======Intensity Time Trace Analysis======
===== Summary =====
This tutor... of the main window.
{{ using_the_intensity_time_trace_script_Image_1.png }}
* Highlight the file ''Cy5_immo_Lifetime_Trace.ptu'' by a single mouse click.
**Note:** This fi
{{tag> tutorial howto FRET PIE analysis time-trace SymPhoTime}}
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====== Calculate Ratiometric S... requires a spectrally resolved fluorescence time trace, in which donor and acceptor have been excited al... rY?large }}
**Note:** The script requires a time trace containing the fluorescence of two spectrally sep... lated yet, this area is still empty.
* In the "Trace Settings" on the left, change the binning to 0.5
{{tag> tutorial howto time-trace analysis FRET SymPhoTime}}
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====== Calculate Ratiometric Singl... requires a spectrally resolved fluorescence time trace. As a result, the distribution of FRET efficienci... sites =====
**Note:** The script requires a time trace containing the fluorescence of two spectrally sep... lated yet, this area is still empty.
* In the "Trace Settings" on the left, change the binning to 0,5
age.\\
- **Upper center/right:** Intensity time trace. The display can be changed using the "Trace Settings" of the analysis options. The large window shows the inset of the complete trace above highlighted in green. The photon counting h... of the different intensity values. Usually, this trace is used to check, whether the signal is stable du
\\
- **Upper center/right:** the intensity time trace. The display option can be changed using the "Trace Settings" of the analysis options. The large window shows the inset of the complete trace above marked in green. The photon counting histog... of the different intensity values. Usually, this trace is used to check, whether the signal is stable du
ary, exhibits a distinct bunching term on its FCS trace originating from photophysical processes, i.e. ci... page.
- **Upper center/right:** Intensity time trace. The display can be changed using the "trace display settings” of the analysis options. The large window shows the inset of the complete trace above highlighted in green. The photon counting h
steps according to the tutorial [[Calculate FCCS Trace with the Grouped FCS Script]].
**Note:** This wa... page.
- **Upper center/right:** Intensity time trace. The display can be changed using the "trace display settings of the analysis options. The large window shows the inset of the complete trace above highlighted in green. The photon counting h
ile up limit the count rate displayed in the Time Trace below the FLIM image is used.
* A red line indi... 0% of the laser repetition rate.
* The **"Time Trace Binning"** should be set to 0.01 ms which equals ... he Leica SP8 SMD. Here, the bin width in the time trace is fixed at 1 msec averaging many pixels. This mi... each pixel is displayed as one point in the time trace. At a pixel dwell time of 1 µs the average of 10
te an antibunching curve from a fluorescence time trace of a single emitter, in this example a nanodiamon... lating an antibunching curve, a fluorescence time trace has to be recorded.\\
To separate the fluorescenc... age.\\
- **Upper center/right:** Intensity time trace. The display can be changed using the "Trace Settings" of the analysis options. The large window shows t
ere.\\
- **Upper center/right:** Intensity time trace. The display option can be changed using the "Trace Settings" of the analysis options. The large window shows the inset of the complete trace above marked in green. The photon counting histog... tensity values.\\
- **Lower center/right:** FCS-trace(s) window. As first the FCS correlation criteria
alhistograms.png | Original data file content }}
Trace #0 shows the IRF, Trace #2 is the decay curve. The other data blocks are empty. Both Trace #0 and #2 will be imported into a single ''.etc''
*For a single immobilized molecule antibunching trace you will need at least a trace of 30s fluorescence emission with a minimum mean countrate of 10 kcps.
w display (Image, FCS-traces, TCSPC-curve or Time Trace - depending on the type of analysis). Additionall... of the crosscorrelation is calculated.
=== Time-Trace ===
* Max. Counts
* Av. Counts
=== TCSPC ===