g>tutorial howto analysis FLIM imaging FRET SymPhoTime}}
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====== FLIM-FRET Calculation for M...
This tutorial shows step-by-step, how the "Lifetime FRET Image" analysis of SymPhoTime 64 can be used to calculate pixel-by-pixel the average [[glossary:fr... average FRET-efficiency, first the donor only lifetime is determined using cells that have only been tra
{{tag>howto analysis lifetime tutorial SymPhoTime}}
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====== SymPhoTime Lifetime Fitting ======
===== Summary =====
This tutorial shows step-by-step, how to fit a selecte
{{tag>howto tutorial FLIM analysis SymPhoTime MicroTime LSM_upgrade}}
====== Lifetime-Fitting Using the FLIM Analysis (updated for SymPhoTime V 2.5 and above) ======
===== Introduction ====
ved.
Such a cocktail cannot be stored for a long time. Latest the KI will photochemically decompose.
=... of course) any of the above mentioned fast decay time fluorophores and record their response. The signa... surement series, provided that the system has had time to warm up (~15min), and neither the repetition r... used as for the sample), and re-used later. Over time or upon changes of the excitation wavelength, the
etectors slightly.
===== Antibunching using SymPhoTime =====
*with [[Products:PicoHarp 300]] in [[g... ossary:T3-mode]]
*not possible with SymPhoTime (32-bit version), because in SPT32 the Sync Divid... 400]] softwares as well as with [[software:SymPhoTime64]], where the sync divider can be disabled.
Usi... *measurements are possible with [[software:SymPhoTime64]]
==== Changing from Normal Acquisition (T3-mo