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===== Background information =====
FLIM-FRET is a robust method to determ... l_donors_Image_9.png }}
**Note:**\\
For more information about the different ROI selection tools, chec... ows program. For this tutorial, a computer with German specific settings was used, therefore, the decim
ease check if they use current versions of file formats and/or programming libraries before you invest ... the advantages of C++ for optimal speed and performance and bridges the gap between the high-speed cap... ose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model fun
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[[doku>DokuWiki]] supports some simple markup language, which tries to... >toolbar|quickbuttons]], too.
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The whole [[#images_and_other_files|image]
lation function =====
In general all relevant information is conveyed by the normalised autocorrelation function, which is defined as the mean product of th... r because of the internal flexibility of the conformation of the biomolecule the quencher comes closer ... orescence one can infer the timescale of the conformational changes of the molecule under study. \\
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the count rate should be kept lower than for a normal FLIM measurement (see [[glossary:Differential Co... ly. Please check the different FLIM-related tutorials for more information about the fitting.
s the image as an ASCII array using a text-file format. Thereby one array encodes the intensity values,... ge series as a series of binary files. The data format of these files is described on the SymPhoTime On... ine help. The binary files contain the photon information of each TCSPC-channel and can be imported e.g... roTime system, or you transfer the image size information automatically via a handshake software, as in