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Tel.: +49-30-12088200\\
E-Mail: info@picoqu
====== Formatting Syntax ======
[[doku>DokuWiki]] supports some simple markup language, which tries to... >toolbar|quickbuttons]], too.
===== Basic Text Formatting =====
DokuWiki supports **bold**, //italic/... ki:dokuwiki-128.png}}]]
Please note: The image formatting is the only formatting syntax accepted in link names.
The whole [[#images_and_other_files|image]
ease check if they use current versions of file formats and/or programming libraries before you invest ... the advantages of C++ for optimal speed and performance and bridges the gap between the high-speed cap... ose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model fun
n/process^
|''.ptu''|Raw data in a unified TTTR-format|<WRAP>
- Generated by a measurement
- Import... nt file, contains manually entered text (e.g. information about the experiment)|Menu: **File → Create C... ) file opens a file viewer, in which you find information about the file type and acquisition settings
<imgcaption image1| ''.ptu'' file information and comment >
{{info_image.png?800| }}
</imgc
lation function =====
In general all relevant information is conveyed by the normalised autocorrelation function, which is defined as the mean product of th... r because of the internal flexibility of the conformation of the biomolecule the quencher comes closer ... orescence one can infer the timescale of the conformational changes of the molecule under study. \\
{{
e FRET pair can be analyzed, e.g. to observe conformational changes.\\
To record such traces, usually s... was used to excite the Cy5 dye directly. This information is used to distinguish molecules with and wit... cules as in this example file. For observing conformational fluctuations of a single immobilized molecu... ning only the acceptor with just the laser line normally used to excite the donor.\\
"Gamma" corrects f
e FRET pair can be analyzed, e.g. to observe conformational changes.\\
To record such traces, usually s... was used to excite the Cy5 dye directly. This information is not used in this script (in fact, for this... cules as in this example file. For observing conformational fluctuations of a single immobilized molecu... ning only the acceptor with just the laser line normally used to excite the donor.\\
**"Gamma"** correc
outube>NRFaEuSb778?large }}
===== Background Information =====
* ATTO488 (ATTO-Tec, Germany) is a suited calibration dye to calibrate the confocal micro... used by detector afterpulsing behave similar as normal background photons.
**Note:** If you want to ch... el and see the fitting parameter. If you
need information about how the fitting models are defined and
ose FCS evaluation software that, amongst other formats, supports the established Zeiss ConfoCor3 ~.fcs file format. PyCorrFit comes with several built-in model fun...
PyCorrFit does support the PicoQuant t3r file format (currently only '''*.pt3''') [[https://github.co... ement request for implementing '''*.ptu''' file format support is existing. [[https://github.com/paulmu
GmbH\\
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Tel: +49 30 1208820 0\\
Fax: +49 30 1208820... te the materials on this website for personal information purposes only. Please note that any product, ... ant GmbH logo are registered trademarks. This information could contain technical inaccuracies or typographical errors, and information will be changed, updated and deleted without
s the image as an ASCII array using a text-file format. Thereby one array encodes the intensity values,... ge series as a series of binary files. The data format of these files is described on the SymPhoTime On... ine help. The binary files contain the photon information of each TCSPC-channel and can be imported e.g... roTime system, or you transfer the image size information automatically via a handshake software, as in
orescence and Fluorescence Microscopy by Nico Stuurman]]
* [[video_tutorials#Kurt Thorn: Optical Sec... es and instrumentation. \\
* Many useful information can be found in [[https://www.microscopyu.com... [[ https://www.microscopyu.com/tutorials/imageformation-airyna |Numerical Aperture and Image Resoluti
y [[http://www.ibiology.org/ibioseminars/nico-stuurman-biography.html|Nico Stuurman]] at UCSF.
{{ youtube>iPrZ84kHH2U?large }}
[[http://www.ibiology.org/ibioseminars/techniques/nico-stuurman-part-1.html]]
==== Confocal Microscopy ====
Ma
easurements always implicitly provide routing information if a router is used. Routing information is represented by four bits in each TTTR record, that all... or maximum throughput, T3 Mode data streams are normally written directly to disk. The current *Harp so
outube>rOas2iDwUzY?large }}
===== Background Information =====
[[glossary:fret|FRET]] is a method to determine molecular interactions or conformational changes based on a suited donor/acceptor pa... and "7" for "Cross Talk".
**Note:** For more information about the equation used for image calculation