s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet... electronics (e.g. hybrid PMA-detector + MultiHarp photon counting board), where the peak count rates have ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve
nfiguration with PicoQuant Time Correlated Single Photon Counting Instruments (TCSPC devices). It harnesse... sampled” signals or on “point-processes”, such as photon timestamps. Pycorrelate allows computing cross-co... on to support the use of a Time-Correlated Single Photon Counting system for application to Positron Annih... Python package to analyze time-correlated single-photon counting (TCSPC) data sets, namely fluorescence l
for fluorescence measurement
</code>
==== Two photon excitation (TPE) ====
Do not attempt to record a... an be adapted.
A special case are systems with 2-Photon-Excitation (2PE). Here, usually TiSa-lasers are u
photons emitted (after the detection of a genuine photon) by one of the SPADs, detected by the second SPAD... o H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/ZnS qua... ting M., Wahl M., Erdmann R: //Recent advances in photon coincidence measurements for photon antibunching and full correlation analysis//. In: //Proceedings of SP
s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\
Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Parameters**\\
Her... f the decay window (often, this is the case for 2-Photon-Excitation data acquired with a TiSa-laser with 8
s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\
Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Parameters**\\
Her
S0-S1 excitation) and cannot emit a fluorescence photon.
{{:general:singlet-triplet-fluctuations.png|}}
... tion spectroscopy by using time-correlated single photon counting. \\ Triffo et al, 2012, "Monitoring Lipi... s of proteome dynamics in living cells"
* **two-photon FCS** \\ Schwille and Heinze, 2001, "Two-Photon cross-correlation spectroscopy"
* **scanning FCS** \\ R
s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\
Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Parameters**\\
Her... in order to decrease processing time and increase photon numbers per pixel, while still visualizing the te
s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\
Here the frequency of photon counts corresponding to the individual mean lifet... ntrols on the right of the plot. The histogram is photon weighted.\\
**4: Decay Fitting Parameters**\\
Her... decay due to limited pulse pair resolution of the photon detector;
Even the best detector cannot deliver i
happens because you correlate the first arriving photon against a photon at the longer time, which will be an increasingly unlikely event because the earliest st... art-stop correlation but the correlation of every photon against every photon.
It can be done using the SymPhoTime Software (http://www.picoquant.com/products/sw
read over the [[glossary:TCSPC]] window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet... ing of 3 to decrease processing time and increase photon numbers per pixel.
{{ visualizing_dynamics_Image_