LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
nfiguration with PicoQuant Time Correlated Single Photon Counting Instruments (TCSPC devices). It harnesse... sampled” signals or on “point-processes”, such as photon timestamps. Pycorrelate allows computing cross-co... on to support the use of a Time-Correlated Single Photon Counting system for application to Positron Annih... Python package to analyze time-correlated single-photon counting (TCSPC) data sets, namely fluorescence l
for fluorescence measurement
</code>
==== Two photon excitation (TPE) ====
Do not attempt to record a... an be adapted.
A special case are systems with 2-Photon-Excitation (2PE). Here, usually TiSa-lasers are u
of the dip, they are however artifacts caused by photons emitted (after the detection of a genuine photon) by one of the SPADs, detected by the second SPAD. The ... o H. C., Huang J., Tang J.: //Antibunching single-photon emission and blinking suppression of CdSe/ZnS qua... ting M., Wahl M., Erdmann R: //Recent advances in photon coincidence measurements for photon antibunching
LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\
Here the frequency of photon counts corresponding to the individual mean lifet
LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet
LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\
Here the frequency of photon counts corresponding to the individual mean lifet
S0-S1 excitation) and cannot emit a fluorescence photon.
{{:general:singlet-triplet-fluctuations.png|}}
... tion spectroscopy by using time-correlated single photon counting. \\ Triffo et al, 2012, "Monitoring Lipi... s of proteome dynamics in living cells"
* **two-photon FCS** \\ Schwille and Heinze, 2001, "Two-Photon cross-correlation spectroscopy"
* **scanning FCS** \\ R
LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\
Here the frequency of photon counts corresponding to the individual mean lifet
LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... s randomly spread over the TCSPC window, the mean photon arrival time of the dark background is usually ve... ence Lifetime Histogram**\\
Here the frequency of photon counts corresponding to the individual mean lifet
happens because you correlate the first arriving photon against a photon at the longer time, which will be an increasingly unlikely event because the earliest stop photons always win and the experiment restarts.
A simul... art-stop correlation but the correlation of every photon against every photon.
It can be done using the S
be entered in the field above. Here we select the photons, which arrive roughly with the laser pulse. The ... e FastFLIM" to apply the time gate on the imaging photons
{{:flim-image_after_gate_selection_2.png|}}}}
... a second time-gated image with the complementary photon selection, just click on the white area in the ga... splay the "Events" and get a image created from the later photons.
{{:gatedimage_latephotons.png|}}
LIM" lifetime, i.e. the mean arrival times of the photons after the laser pulse. When not defined otherwis... ximum. As the mean arrival time of the background photons in the areas where no fluorescence is detected, ... read over the [[glossary:TCSPC]] window, the mean photon arrival time of the dark background is usually ve... escence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifet