gen Vorfalls von der Löschung ausgenommen.
**6. Routinemäßige Löschung und Sperrung von personenbezoge... cherfrist ab, werden die personenbezogenen Daten routinemäßig und entsprechend den gesetzlichen Vorschr... schutzbestimmungen zu Einsatz und Verwendung von YouTube**
Der für die Verarbeitung Verantwortliche hat auf dieser Internetseite Komponenten von YouTube integriert. YouTube ist ein Internet-Videoport
ank lines. If you want to **force a newline** without a paragraph, you can use two backslashes followed...
or followed by\\ a whitespace \\this happens without it.
This is some text with some linebreaks\\ N... or followed by\\ a whitespace \\this happens without it.
You should use forced newlines only if reall... by using a colon in the pagename.
For details about namespaces see [[doku>namespaces]].
Linking to a
T3 Mode measurements always implicitly provide routing information if a router is used. Routing information is represented by four bits in each TTTR record, that allow up to four routing channels to be distinguished. However, these b
Ch.0.
-Disconnect SPAD 1 Signal from PHR800 Router.
-Connect SPAD 1 Signal to [[Products:Pico... is NIM compatible. If the SPAD provides only TTL output pulses, you need to use the SIA400 adapter.
... ors directly to the [[Products:PicoHarp 300]] without passing SPAD 2 through the router. **In this case, however, the router cable (DVI-cable) needs to be d
=
This page is a collection of lectures found on youtube that explain the basics of [[wp>fluorescence]]... rman-biography.html|Nico Stuurman]] at UCSF.
{{ youtube>iPrZ84kHH2U?large }}
[[http://www.ibiology.o... nents of confocal microscopes and their use.
{{ youtube>BUSZD4IVYSw?large }}
This video is part of a s... eos. This is the related playlist: [[https://www.youtube.com/playlist?list=PLO0yDSkagDljzI0bdM5m1njrdLd
which can be used to detect subpopulations.
{{ youtube>cSAbgJpZhrY?large }}
**Note:** The script req... ion is used to distinguish molecules with and without acceptor dyes and to filter the events for signals without acceptor to calculate the stoichiometry parameter... n intensity threshold is calculated for filtering out events without acceptor present. Four the fourth
the detection volume. As molecule diffuse in and out the relative fluctuations of the fluorescent inte... sity are large (no intensity when the molecule is out, high intensity when the molecule is in) in compa... ox 80%>
The above equation can give you an idea about how big differences
in diffusional correlation t... nal as input and give the correlation curve as an output.
{{:general:correlator_black_box2.png?550|}}
t record is generated that contains information about the channel it came from and the arrival time of ... the channel number and 28 bits for the time-tag. Routing is not supported. If the time tag overflows, a... records are queued in a [[FIFO]] (First In First Out) buffer capable of holding up to 256 k event reco... those lost in the [[dead time]] anyhow. The FIFO output is continuously read by the host PC, thereby m
cy using the "Lifetime FRET Image" analysis.
{{ youtube>zHfGae_Pd8A?large }}
===== Background informa... mage_9.png }}
**Note:**\\
For more information about the different ROI selection tools, check the tuto... of importance. Therefore exclude the bright dots outside the nucleus of the cell on the lower right by... ously "CRTL" and draw rectangles around the spots outside the nucleus.
* **Response:** The marked reg
phere or contamination of the sample holder.
{{ youtube>JLiDnlS5BS8?large }}
===== Step-by-Step =====... _at_14-35-37.jpg?600 |}}
* Select either IN or OUT position
**Note:** The sample holder has two inde... on beam will directly hit the sample, when in the OUT position the sample will only be illuminated indi... der into the IN-position.
* Select either IN or OUT position.
**Note:** The position should be the s
ontains manually entered text (e.g. information about the experiment)|Menu: **File → Create Comment**|D... ns a file viewer, in which you find information about the file type and acquisition settings
<imgcapti... line-analysis will contain relevant information about the measurement. To check the values calculated d... r above the scale, right click and select **"Clip Outliners"**. This feature ignores outliners of the p
tandard TTL signals ? ). \\
Check the type of the output connector and check which pin of the output connector delivers which marker.
What type of signal is... 0-0.8 V for a logic "low"-level. For more info about TTL signals see [[ http://www.allaboutcircuits.com/textbook/digital/chpt-3/logic-signal-voltage-level
ed in three different colors for each pixel.
{{ youtube>L68IgRbNSLE?large }}
==== Open an Image ====
... iversity Map//. This new feature tells you more about the different populations in the picture. The //D... e second therefore is the non-FRET population without any FRET performing molecules.
{{ pattern_matchin... the residuals of the initial fit show no trend or outliers, it is safe to assume that the selected patt
erest are defined which are finally fitted.
{{ youtube>g6uyvLQW4nY?large }}
===== Step-by-Step =====... ry selection, the corresponding red dot is greyed out.
{{ roi_fitting_using_the_flim_script_Image_14.pn... image are highlighted, everything else is greyed out. The decay corresponding to ROI 2 contains now th... e is usually a bit arbitrary.\\
For explanation about the fitting model and the used equations, click o