ank lines. If you want to **force a newline** without a paragraph, you can use two backslashes followed...
or followed by\\ a whitespace \\this happens without it.
This is some text with some linebreaks\\ N... or followed by\\ a whitespace \\this happens without it.
You should use forced newlines only if reall... by using a colon in the pagename.
For details about namespaces see [[doku>namespaces]].
Linking to a
ion is used to distinguish molecules with and without acceptor dyes and to filter the events for signals without acceptor to calculate the stoichiometry parameter... n intensity threshold is calculated for filtering out events without acceptor present. Four the fourth trace, also an intensity threshold is calculated, whi
the detection volume. As molecule diffuse in and out the relative fluctuations of the fluorescent inte... sity are large (no intensity when the molecule is out, high intensity when the molecule is in) in compa... ox 80%>
The above equation can give you an idea about how big differences
in diffusional correlation t... only translational diffusion of the emitters without any additional processes going on. In such a case
_at_14-35-37.jpg?600 |}}
* Select either IN or OUT position
**Note:** The sample holder has two inde... on beam will directly hit the sample, when in the OUT position the sample will only be illuminated indi... der into the IN-position.
* Select either IN or OUT position.
**Note:** The position should be the s
t record is generated that contains information about the channel it came from and the arrival time of ... records are queued in a [[FIFO]] (First In First Out) buffer capable of holding up to 256 k event reco... t rate over a long period of time exceeds the readout speed of the PC, a FIFO overrun could occur. In c
records are queued in a [[FIFO]] (First In First Out) buffer capable of holding up to 256 k event reco... t rate over a long period of time exceeds the readout speed of the PC, a FIFO overrun could occur. In c
ry selection, the corresponding red dot is greyed out.
{{ roi_fitting_using_the_flim_script_Image_14.pn... image are highlighted, everything else is greyed out. The decay corresponding to ROI 2 contains now th... e is usually a bit arbitrary.\\
For explanation about the fitting model and the used equations, click o
ontains manually entered text (e.g. information about the experiment)|Menu: **File → Create Comment**|D... ns a file viewer, in which you find information about the file type and acquisition settings
<imgcapti... line-analysis will contain relevant information about the measurement. To check the values calculated d... in before the acquisition (see online tutorials about FLIM and FLIM-FRET imaging)
===== Working wit
ors directly to the [[Products:PicoHarp 300]] without passing SPAD 2 through the router. **In this case... displayed.
====== Some MT200 based literature about Antibunching ======
-Yuan C. T., Yu P., Ko H
not necessary to calculate first a FCS curve without the photon filter. You can also directly activate... the FCS Calibration Script]] for a description
about how to calibrate the confocal volume.\\
If no cal... the fitting parameter. If you
need information about how the fitting models are defined and what is th