ion is used to distinguish molecules with and without acceptor dyes and to filter the events for signals without acceptor to calculate the stoichiometry parameter... n intensity threshold is calculated for filtering out events without acceptor present. Four the fourth trace, also an intensity threshold is calculated, whi
_at_14-35-37.jpg?600 |}}
* Select either IN or OUT position
**Note:** The sample holder has two inde... on beam will directly hit the sample, when in the OUT position the sample will only be illuminated indi... der into the IN-position.
* Select either IN or OUT position.
**Note:** The position should be the s
ry selection, the corresponding red dot is greyed out.
{{ roi_fitting_using_the_flim_script_Image_14.pn... image are highlighted, everything else is greyed out. The decay corresponding to ROI 2 contains now th... e is usually a bit arbitrary.\\
For explanation about the fitting model and the used equations, click o
ontains manually entered text (e.g. information about the experiment)|Menu: **File → Create Comment**|D... ns a file viewer, in which you find information about the file type and acquisition settings
<imgcapti... line-analysis will contain relevant information about the measurement. To check the values calculated d... in before the acquisition (see online tutorials about FLIM and FLIM-FRET imaging)
===== Working wit
ors directly to the [[Products:PicoHarp 300]] without passing SPAD 2 through the router. **In this case... displayed.
====== Some MT200 based literature about Antibunching ======
-Yuan C. T., Yu P., Ko H
not necessary to calculate first a FCS curve without the photon filter. You can also directly activate... the FCS Calibration Script]] for a description
about how to calibrate the confocal volume.\\
If no cal... the fitting parameter. If you
need information about how the fitting models are defined and what is th
mage_9.png }}
**Note:**\\
For more information about the different ROI selection tools, check the tuto... th donor and acceptor construct and two cells without. For the cells with the FRET donor only, a FRET e... nce requires some effort and distinct knowledge about the sample. A few aids are available on the lab.
s, and it is very likely to produce artefacts without allowing to tell apart artefacts and effects.
==... lso a remarkably short fluorescence lifetime of about 10 ps. This short lifetime does not depend on the
scribed here can also be used for IRF-fitting without time gating. However, as these decays contain the... ly. Please check the different FLIM-related tutorials for more information about the fitting.
h a TiSa-laser with 80MHz repetition rate). Check out the rapidReconvolution tutorial for this case (ht... d_reconvolution_algorithm).\\
For explanations about the fitting model and the used equations, click o... **\\
For pixel-by-pixel fits, we are interested about how the lifetime parameters are distributed in th