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howto:visualizing_dynamics_with_the_multi_frame_flim_analysis

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howto:visualizing_dynamics_with_the_multi_frame_flim_analysis [2015/11/03 15:32]
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howto:visualizing_dynamics_with_the_multi_frame_flim_analysis [2015/11/03 15:33] (current)
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   - **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifetimes is plotted. The settings of the plot can be adapted using the controls on the right of the plot.\\   - **Upper right:** Fluorescence Lifetime Histogram. Here the frequency of photon counts corresponding to the individual mean lifetimes is plotted. The settings of the plot can be adapted using the controls on the right of the plot.\\
   - **Lower left:** Lifetime fitting parameters allowing to define the lifetime fitting models. Fitting is then applied to the lifetime graph(s) in the [[glossary:​TCSPC]] histogram.\\   - **Lower left:** Lifetime fitting parameters allowing to define the lifetime fitting models. Fitting is then applied to the lifetime graph(s) in the [[glossary:​TCSPC]] histogram.\\
-  - **Lower center/​left:​** TCSPC histogram of all photons in the image in green (= dataset 0) and the [[glossary:​TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation of the instrument response function (= IRF) is shown. The IRF reconstruction is deducted from the rising edge of the TCSPC histogram.+  - **Lower center/​left:​** ​[[glossary:TCSPC]] histogram of all photons in the image in green (= dataset 0) and the [[glossary:​TCSPC]] histogram of a single pixel in grey (= dataset 1). In red an estimation of the instrument response function (= [[glossary:IRF]]) is shown. The [[glossary:IRF]] reconstruction is deducted from the rising edge of the [[glossary:TCSPC]] histogram.
  
   * To enhance the lifetime contrast within the image, adapt the color scale from 0 to 15 ns by typing these values as limits for the Fast Lifetime color scale. Also adjust the intensity scale to 1 – 200 counts.   * To enhance the lifetime contrast within the image, adapt the color scale from 0 to 15 ns by typing these values as limits for the Fast Lifetime color scale. Also adjust the intensity scale to 1 – 200 counts.
howto/visualizing_dynamics_with_the_multi_frame_flim_analysis.txt · Last modified: 2015/11/03 15:33 by admin