howto:using_the_anisotropy_image_script
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howto:using_the_anisotropy_image_script [2018/04/12 09:39] – [Select a file and start the script] doerr | howto:using_the_anisotropy_image_script [2018/04/12 11:44] (current) – [Select a file and start the script] doerr | ||
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- **Left:** Imaging analysis and processing options. | - **Left:** Imaging analysis and processing options. | ||
- **Upper center:** Fluorescence intensity image, displayed in false color scale. The perpendicular and parallel | - **Upper center:** Fluorescence intensity image, displayed in false color scale. The perpendicular and parallel | ||
- | - **Upper right:** Fluorescence anisotropy image. By default, the rainbow color scale is spread between -0.4 and 1 (for single molecules and aligned molecules the anisotropy can vary between -0.5 and 1). When measuring a randomly aligned ensemble with parallel excitation and emission dipole moments, the anisotropy values can theoretically spread between 0 (very fast rotation or extremely efficient HOMO-FRET) and 0.4 (rotation significantly slower than the fluorescence lifetime, no HOMO-FRET). | + | - **Upper right:** Fluorescence anisotropy image. By default, the rainbow color scale is spread between -0.4 and 1. |
- **Lower graph:** Anisotropy histogram over the different pixels. | - **Lower graph:** Anisotropy histogram over the different pixels. | ||
+ | |||
+ | **Note:** | ||
+ | The anisotropy values can theoretically spread between -0.5 and 1 for perpendicular and parallel excitation and emission dipoles respectively. The anisotropy value of 0 is expected in case of very fast rotation or extremely efficient energy transfer processes | ||
==== Adapt the script to the image and the system parameters ==== | ==== Adapt the script to the image and the system parameters ==== |
howto/using_the_anisotropy_image_script.txt · Last modified: 2018/04/12 11:44 by doerr