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howto:separation_of_2_species_with [2014/06/18 12:57]
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howto:separation_of_2_species_with [2014/06/18 14:22] (current)
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 {{tag> howto tutorial FCS FLCS analysis SymPhoTime}} {{tag> howto tutorial FCS FLCS analysis SymPhoTime}}
  
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 ====== Separation of 2 Species with Different Lifetimes Using FLCS ====== ====== Separation of 2 Species with Different Lifetimes Using FLCS ======
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 This tutorial shows step-by-step,​ how the FLCS analysis can be used to calculate separate autocorrelation curves for the two components of a mixture of ATTO655 and Cy5 based on their different lifetimes. This tutorial shows step-by-step,​ how the FLCS analysis can be used to calculate separate autocorrelation curves for the two components of a mixture of ATTO655 and Cy5 based on their different lifetimes.
  
 +{{ youtube>​xx-WzT5TgqA?​large }}
  
 ===== Background Information ===== ===== Background Information =====
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   * Click on "Save result"​ to save this curve. Rename the generated file as "​Cy5-FLCS"​.   * Click on "Save result"​ to save this curve. Rename the generated file as "​Cy5-FLCS"​.
   * These curves can now be fitted by selecting "​Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:​Calculate and Fit FCS Traces with the FCS Script]].   * These curves can now be fitted by selecting "​Transfer to fit". The process of how to fit a FCS curve is explained in the tutorial [[howto:​Calculate and Fit FCS Traces with the FCS Script]].
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howto/separation_of_2_species_with.txt ยท Last modified: 2014/06/18 14:22 by admin