howto:separation_of_2_species_with
Differences
This shows you the differences between two versions of the page.
Both sides previous revisionPrevious revisionNext revision | Previous revision | ||
howto:separation_of_2_species_with [2014/05/06 14:39] – admin | howto:separation_of_2_species_with [2014/06/18 12:22] (current) – admin | ||
---|---|---|---|
Line 1: | Line 1: | ||
- | {{tag> howto tutorial FCS FLCS analysis}} | + | {{tag> howto tutorial FCS FLCS analysis |
- | ~~TOC~~ | + | ~~NOTOC~~ |
- | ====== Separation of 2 Species with Different Lifetimes Using the FLCS Analysis | + | ====== Separation of 2 Species with Different Lifetimes Using FLCS ====== |
Line 10: | Line 10: | ||
This tutorial shows step-by-step, | This tutorial shows step-by-step, | ||
+ | |||
+ | {{ youtube> | ||
===== Background Information ===== | ===== Background Information ===== | ||
- | |||
- | **Note:**\\ | ||
The two fluorophores ATTO655 (ATTO-Tec, Germany) and Cy5 (GE Healthcare) have almost identical spectral properties and are thus excited with the same wavelength (e.g. 635 nm) and detected at the same spectral range. As also their molecular weight is comparable, the diffusion constant is too similar to distinguish between both dyes in a mixture by means of standard FCS.\\ | The two fluorophores ATTO655 (ATTO-Tec, Germany) and Cy5 (GE Healthcare) have almost identical spectral properties and are thus excited with the same wavelength (e.g. 635 nm) and detected at the same spectral range. As also their molecular weight is comparable, the diffusion constant is too similar to distinguish between both dyes in a mixture by means of standard FCS.\\ | ||
Nevertheless, | Nevertheless, | ||
Line 218: | Line 218: | ||
* Click on "Save result" | * Click on "Save result" | ||
* These curves can now be fitted by selecting " | * These curves can now be fitted by selecting " | ||
- | |||
- |
howto/separation_of_2_species_with.txt · Last modified: 2014/06/18 12:22 by admin