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--- howto:flim-fret_measurement_using_an_olympus_fv1200_with_a_flim_and_fcs_upgrade [2014/06/19 08:49] – admin
+++ howto:flim-fret_measurement_using_an_olympus_fv1200_with_a_flim_and_fcs_upgrade [2015/02/06 11:55] – admin
@@ Line 1: / Line 1: @@
-{{tag> video howto demo Olympus FV1200 LSM_upgrade}}
+{{tag> video FLIM FRET acquisition howto demo Olympus FV1200 LSM_upgrade}}
 ====== FLIM-FRET Measurement using an Olympus FV1200 with a FLIM and FCS upgrade ======
-<WRAP center important 80%>
-DRAFT
-</WRAP>
+This tutorial shows the recording of FLIM-FRET images using an LSM upgrade kit, in this case connected to an Olympus FluoView FV1200 laser scanning microscope.
+In FLIM-FRET measurements, the fluorescence lifetime of a FRET-donor in presence or absence of the acceptor is measured. This can be used
+to monitor interactions between two molecules via FRET.
+The principles are similar as for any FLIM-recording.
+General recording of FLIM images can also be found using the link below.
+The general principles of the data acquisition are analogous in all LSM upgrade kits.
+The movie was recorded in 02/2014; the recording procedure may be slightly different in older or newer
+models.
 {{ youtube>YIt5GjA_we0?large }}
@@ Line 14: / Line 20: @@
  {{topic>howto +IRF &nodate&nouser}}
  {{topic>howto +FLIM +FRET +analysis &nodate&nouser}}
+ {{topic>howto +FLIM +Nikon}}