howto:flim-fret_calculation_for_single_exponential_donors
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howto:flim-fret_calculation_for_single_exponential_donors [2020/05/05 15:39] – [Step-by-Step Tutorial] ruckelshausen | howto:flim-fret_calculation_for_single_exponential_donors [2020/05/06 09:38] (current) – [Step-by-Step Tutorial] ruckelshausen | ||
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* On top left, under Region of Interest, check "Use ROI" box and click on "ROI from Threshold" | * On top left, under Region of Interest, check "Use ROI" box and click on "ROI from Threshold" | ||
- | + | {{ :howto:flim-fret-1expd_roiactive.png?300 |}} | |
- | {{ :writingroom:roiactive.png?300 |}} | + | |
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* In this example set the lower intensity threshold to 500 counts. | * In this example set the lower intensity threshold to 500 counts. | ||
+ | {{ : | ||
* Click " | * Click " | ||
**Response: | **Response: | ||
- | {{ : | + | {{ : |
* In the lifetime fitting panel, keep the default Fitting model " | * In the lifetime fitting panel, keep the default Fitting model " |
howto/flim-fret_calculation_for_single_exponential_donors.txt · Last modified: 2020/05/06 09:38 by ruckelshausen